Poorly processed reusable surface disinfection tissue dispensers may be a source of infection

BMC Infectious Diseases

Table 2 Number of CFU per mL of dispenser isolates transferred in fresh surface disinfectant solution at room temperature

Product at a dilution to be effective in 60 minutes	Active ingredient(s) of undiluted product; concentration (w/w)	Type of cells	Dispenser	CFU/mL
Product at a dilution to be effective in 60 minutes		Type of cells	Dispenser	Day 7	Day 14	Day 21	Day 28
Mikrobac forte (0.5%)	Benzyl-C12-18-alkyldimethylammoniumchloride (19.9%), N-(3-Aminopropyl)-N-dodecylpropan-1,3-diamin (5%)	Adapted cells	1	10⁶	10⁷	n.a.	n.a.
			2	10⁶	10⁷	n.a.	n.a.
			3	10⁶	10⁷	n.a.	n.a.
		De-adapted cells	1	10⁷	10⁷	n.a.	n.a.
			2	10⁷	10⁷	n.a.	n.a.
			3	10⁷	10⁷	n.a.	n.a.
		ATCC 27061	1	0	0	0	0
			2	0	0	0	0
			3	0	0	0	0
Kohrsolin FF (0.5%)	Glutaral (5%), Benzyl-C12-18-alkyldimethylammoniumchloride (3%), Didecyldimethylammoniumchlorid (3%)	Adapted cells	1	0	0	0	0
			2	0	0	0	0
			3	0	0	0	0
		De-adapted cells	1	0	0	0	0
			2	0	0	0	0
			3	0	0	0	0
Terralin protect (0.5%)	Quaternary ammonium compound, Benzyl-C12-16-alkyl-dimethylchloride (22%), 2-Phenoxyethanol (17%), Aminoalkylglycine (0.9%)	Adapted cells	1	60	10⁷	n.a.	n.a.
			2	45	10⁷	n.a.	n.a.
			3	35	10⁷	n.a.	n.a.
		De-adapted cells	1	10⁷	10⁷	n.a.	n.a.
			2	10⁷	10⁷	n.a.	n.a.
			3	10⁷	10⁷	n.a.	n.a.
		ATCC 27061	1	0	0	0	0
			2	0	0	0	0
			3	0	0	0	0
Incidin plus (0.5%)	Glucoprotamin (26%)	Adapted cells	1	10³	10⁷	n.a.	n.a.
			2	10⁷	10⁷	n.a.	n.a.
			3	10³	10⁷	n.a.	n.a.
		De-adapted cells	1	10⁷	10⁷	n.a.	n.a.
			2	10⁷	10⁷	n.a.	n.a.
			3	10⁷	10⁷	n.a.	n.a.
		ATCC 27061	1	0	0	0	0
			2	0	0	0	0
			3	0	0	0	0

Achromobacter species 3 (passage 0 as adapted cells, and passage 5 as de-adapted cells) or Alcaligenes xylosoxidans ATCC 27061 after up to 28 days in a freshly prepared disinfectant solution (2.5 l) with tissues, prepared in a fresh dispenser which was first contaminated with 25 ml of inoculum (approximately 10⁷ CFU per mL).
n.a.: not available.

ISSN: 1471-2334