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Table 2 Number of CFU per mL of dispenser isolates transferred in fresh surface disinfectant solution at room temperature

From: Poorly processed reusable surface disinfection tissue dispensers may be a source of infection

Product at a dilution to be effective in 60 minutes Active ingredient(s) of undiluted product; concentration (w/w) Type of cells Dispenser CFU/mL
Day 7 Day 14 Day 21 Day 28
Mikrobac forte (0.5%) Benzyl-C12-18-alkyldimethylammoniumchloride (19.9%), N-(3-Aminopropyl)-N-dodecylpropan-1,3-diamin (5%) Adapted cells 1 106 107 n.a. n.a.
2 106 107 n.a. n.a.
3 106 107 n.a. n.a.
De-adapted cells 1 107 107 n.a. n.a.
2 107 107 n.a. n.a.
3 107 107 n.a. n.a.
ATCC 27061 1 0 0 0 0
2 0 0 0 0
3 0 0 0 0
Kohrsolin FF (0.5%) Glutaral (5%), Benzyl-C12-18-alkyldimethylammoniumchloride (3%), Didecyldimethylammoniumchlorid (3%) Adapted cells 1 0 0 0 0
2 0 0 0 0
3 0 0 0 0
De-adapted cells 1 0 0 0 0
2 0 0 0 0
3 0 0 0 0
Terralin protect (0.5%) Quaternary ammonium compound, Benzyl-C12-16-alkyl-dimethylchloride (22%), 2-Phenoxyethanol (17%), Aminoalkylglycine (0.9%) Adapted cells 1 60 107 n.a. n.a.
2 45 107 n.a. n.a.
3 35 107 n.a. n.a.
De-adapted cells 1 107 107 n.a. n.a.
2 107 107 n.a. n.a.
3 107 107 n.a. n.a.
ATCC 27061 1 0 0 0 0
2 0 0 0 0
3 0 0 0 0
Incidin plus (0.5%) Glucoprotamin (26%) Adapted cells 1 103 107 n.a. n.a.
2 107 107 n.a. n.a.
3 103 107 n.a. n.a.
De-adapted cells 1 107 107 n.a. n.a.
2 107 107 n.a. n.a.
3 107 107 n.a. n.a.
ATCC 27061 1 0 0 0 0
2 0 0 0 0
3 0 0 0 0
  1. Achromobacter species 3 (passage 0 as adapted cells, and passage 5 as de-adapted cells) or Alcaligenes xylosoxidans ATCC 27061 after up to 28 days in a freshly prepared disinfectant solution (2.5 l) with tissues, prepared in a fresh dispenser which was first contaminated with 25 ml of inoculum (approximately 107 CFU per mL).
  2. n.a.: not available.