SDS-PAGE and immunoblotting analysis of the purified rSEPs. Eleven rSEPs were analyzed with SDS-PAGE (A) and immunoblotted with acute-phase sera (APS) or convalescent-phase sera (CPS) of patient 8 (B). The relative amount of specific antibodies to individual rSEPs in APS or CPS of patient 8 was calculated in microarray assay (C). Lanes 1 to 11 refer to rGroEL, rOmpA-2, rOmpB-3, rPrsA, rRh054_02285, rRplA, rRplY, rRpsB, rSdhB, rSurA, and rYbgF, respectively. Lane M refers to protein markers and their relative molecular masses are indicated in kDa on the left.