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Table 3 P. aeruginosa MLST data from the P. aeruginosa MLST database website (13 th December 2012) and associated SNP profiles for STs of national and international importance

From: A comparison of two informative SNP-based strategies for typing Pseudomonas aeruginosa isolates from patients with cystic fibrosis

20 SNP profilea

MLSTb

Total STs

Related STs

(SLV or DLVc)

CGTCGACTACCTCCCCGGTA

649(AUST-01) d

1

 

TGCAAGCTACCTTCCCGGTA

775(AUST-02)

1

 

TGCAAGCTCCCCTTTGGGCG

242(AUST-03), 996

2

2/2 = SLVs

TGCCGGCTATCCCCCCGACA

787, 788(AUST-04)

2

2/2 = SLVs

TGTCGGCTACCTTTCGGGTA

209, 268, 274(AUST-05,-09, -18,-25, &-31) , 466, 546, 781(AUST-05), 936, 1043(AUST-09), 1068, 1089, 1301, 1326

12

12/12 = SLVs

CGCAAGCTATCCCCCGGGTG

4, 801 (AUST-06), 1292

3

2/3 = SLVs

CGCAAGCTATCCCCCCGGCA

262(AUST-07), 774, 1165

3

3/3 = SLVs

CGCAGGGCCCCCTTCGGGCG

782(AUST-08), 783(AUST-08), 784(AUST-08), 785(AUST-08)

4

4/4 = SLVs

CGCCGGCTCCCCCCCCGACA

179(AUST-10,-12,-14,&-26), 180, 353

3

3/3 = SLVs

TGCAAGCTACCCCCTGGACA

13, 155(AUST-10,-14, -19, &-37), 280, 541, 579, 677, 786(AUST-19), 1276, 1316, 1335

10

10/10 = SLVs

CGCAAGCTACCTCCCCGGTA

384, 1037(AUST-11)

2

 

CGCAGGCTACCTCCTGGGTG

508(AUST-11), 937

2

2/2 = SLVs

TGCCGGCTCCCCCCTGGGCA

554, 804(AUST-11)

2

2/2 = SLVs

CGCAGGCTACCTCCCCGGTA

589, 791, 803(AUST-11)

3

3/3 = SLVs

TGCCGGCTATCCCCCCGGCA

822(AUST-11), 1239(M18)

2

2/2 = SLVs

CGCAAGCTACCTCCCCAGTA

882(AUST-11), 1151, 1233

3

3/3 = SLVs

CGCAGGCTACCTTCCCGGTA

1034(AUST-11)

1

 

CGCAAGGTACCTCCTGGGCG

800(AUST-13)

1

 

CGCAAGGTCCCCCCCGGGTG

389(AUST-13)

1

 

CGTCGGCTATCCTTCCGGTA

17(AUST-15 & Clone C), 318, 322, 380, 636, 688, 845, 958, 1255, 1313

10

9/10 = SLVs

CGCAAGCTACTCTCCCGGTG

905(AUST-16), 1039

2

 

CGTCGGCTATCCCCTGGGCA

398, 399, 401, 810(AUST-17)

4

3/4 = SLVs

CGTCGGCCACTCTTCCGGCG

655(AUST-20), 709

2

2/2 = SLVs

TGCAAGCTACCCTCCCGGTA

669, 808(AUST-21)

2

2/2 = SLVs

CGCCGGGCCTCCTCTGAGTG

809(AUST-22)

1

 

TGTCGGCTACCTTCCCGGTA

833(AUST-23), 839

2

2/2 = SLVs

TACCAGGCCCCCTCCGAGTG

89, 307, 308(AUST-24), 662, 1028

5

2/5 = DLVs

TGTCGGCCCCCCTTCGGGTA

455(AUST-27)

1

 

CGCAAGCTACCTTCCCGGTA

232, 241(AUST-28), 247, 379, 471, 577

6

5/6 = SLVs

CGTCGGCTATCCCCTGGGTA

169, 261(AUST-29)

2

 

CGCAGACTCCCCTCCCGGTA

1036(AUST-30)

1

 

TGCAAGCTATCCTCCCAGCG

236(AUST-32), 239, 240

3

3/3 = SLVs

TGCAAGCTATCCCCCCGGTG

12(AUST-33)

1

 

CGCAAGCTCCCCCCCGGGTA

103, 244, 441, 462, 464, 594, 766, 986, 1038(AUST-34), 1181, 1227, 1338

12

10/12 = SLVs or DLVs

CGCAAGCTATCCTTCCGGTA

445, 553(AUST-35)

2

 

CGCAAGCTCCTCTTTCGGTA

277(AUST-36), 364, 1128, 1390

4

4/4 = SLVs

TGTCGGCTCCTCTTTGGGTA

254(AUST-38), 1041(AUST-38)

2

2/2 = SLVs

TGTCGGCTACCTCCCCGGTG

146(LES), 374, 467, 681, 683, 970

6

6/6 = SLVs

TGCAAGCTACCTCCCCGACG

217(Manchester), 1134

2

2/2 = SLVs

CGCAAGCTACCTCCCCGGCG

386(DK2), 1244

2

 

TGCAGGCTCCCCCCCCAGCA

549(PA01), 1331

2

2/2 = SLVs

TATCGGGCCCCCTCCGAGTG

65, 107, 109, 253(PA14), 297, 338, 342, 377, 532, 773, 815, 923, 1110, 1363

14

3/14 = SLVs or DLVs

CGTCGGCTCTCCCCTGGACA

1394(PACS2)

1

 

CGCTAGGCCCCCTCCGAGTG

227, 230, 235(NCGM2.S1), 533, 534, 696, 745, 976, 989

9

9/9 = SLVs

CGCGGAGCCCTCTCCGTGTG

366, 368, 1006, 1063, 1190, 1191, 1195(PA7)

7

4/7 = SLVs

CGTCGGCTACCTCCCCGACA

406(Dutch-1), 484, 519, 536, 547, 575, 608, 1214, 1235, 1312, 1318

11

10/11 = SLVs or DLVs

CGTCGGCTATCCTTTGGGTA

497(Dutch-2), 544, 895, 1317

4

4/4 = SLVs or DLVs

CGCAAGCTATCCCCCGAGTA

138, 140, 148(Midlands), 956

4

2/4 = SLVs

  1. The STs consistent with recognised P. aeruginosa strains are indicated in parentheses.
  2. aSNP profile is in the order of 7, 45, 322, 381, 387, 416, 488, 881, 894, 937, 1086, 1152, 1297, 1465, 1865, 1958, 2169, 2208, 2337 and 2551. These SNPs were derived from the sequence data from the Pseudomonas aeruginosa MLST database website (http://darwin.phyloviz.net/ComparingPartitions/index.php?link=Tool) on 13 December 2012. bMLST types available from the Pseudomonas aeruginosa MLST database website (http://pubmlst.org/paeruginosa) on 13 December 2012. cSLV: single locus variant, DLV: double locus variant. dBoldface type represents previously characterised National and International MLST types of importance (e.g., AUST-01 has the MLST type 649).
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BMC Infectious Diseases

ISSN: 1471-2334

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