|
SNP sitea
|
Gene target
|
UEP (5′-3′)
|
Mass
|
EP1, mass
|
EP2, mass
|
EP3, mass
|
EP4, mass
|
|---|
|
7
|
acsA
|
ACATCGTCTATGGCCCG
|
5146.4
|
C,5393.5
|
T,5473.5
| | |
|
45
|
acsA
|
ggggTCTGTTCGAGGGCGT
|
5931.8
|
A,6203
|
G,6219
| | |
|
322
|
acsA
|
gggtaGCGTGGGCGCCCGGCA
|
6529.2
|
T,6800.4
|
C,6816.4
| | |
|
381
|
ascA
|
ttcccAGCCGTTCTTCGGCGTGGT
|
7302.7
|
C,7549.9
|
A,7573.9
|
G,7589.9
|
T,7629.8
|
|
387
|
ascA
|
gaaGAGGTTGTCCACCAG
|
5548.6
|
G,5795.8
|
A,5875.7
| | |
|
416b
|
aroE
|
TGCCGTTCAAGGAAGA
|
4930.2
|
C,5177.4
|
A,5201.4
|
G,5217.4
| |
|
aGgCGTTCAAGGAACT
|
|
488
|
aroE
|
ccCACCCTGATCCGCCT
|
5027.3
|
C,5274.5
|
G,5314.5
|
T,5354.4
| |
|
881
|
aroE
|
gagggTGTATGCCAAGGAACCGAC
|
7451.9
|
C,7699
|
G,7739.1
|
T,7778.9
| |
|
894
|
guaA
|
gTCCTCCAAGGTCCTGCT
|
5426.5
|
C,5673.7
|
A,5697.7
|
G,5713.7
| |
|
937
|
guaA
|
tgtTCcgCGATGGCCTTGTGCA
|
6733.4
|
T,7004.6
|
C,7020.6
| | |
|
1086
|
guaA
|
gtCGAGGACAAGTTCCTCGG
|
6158
|
C,6405.2
|
G,6445.2
|
T,6485.1
| |
|
1152
|
guaA
|
ctcaGCACCTTCATCGAAGT
|
6036.9
|
C,6284.1
|
G,6324.2
|
T,6364
| |
|
1297
|
mutL
|
GTGGAAAGCCACGTCGAA
|
5557.6
|
T,5828.8
|
C,5844.8
| | |
|
1465
|
mutL
|
gggCGGCtcGCACCTGTGGGG
|
6520.2
|
C,6767.4
|
T,6847.3
| | |
|
1865
|
nuoD
|
agagaCAGTCtcGGCACAGTTTCAT
|
7666
|
C,7913.2
|
T,7993.1
| | |
|
1958
|
nuoD
|
tgctGATCACGTCGACCCGCTG
|
6687.3
|
G,6934.5
|
C,6974.5
| | |
|
2169
|
ppsA
|
ccggaGCGCTGGCCGATGGCACG
|
7091.6
|
G,7338.8
|
T,7362.8
|
C,7378.8
|
A,7418.7
|
|
2208
|
ppsA
|
CCGACACGTCGTTGATCAC
|
5748.7
|
G,5995.9
|
A,6075.8
| | |
|
2337
|
ppsA
|
CGCCGCGTGGCAGGT
|
4610
|
T,4881.2
|
C,4897.2
| | |
|
2551
|
trpE
|
CAGATCCTGCTCCAG
|
4512.9
|
G,4760.1
|
C,4800.2
|
A,4840
| |
- Non-template bases are indicated in lower case. Mass (Daltons) is provided for the unextended extension primer (UEP), as well as associated extension products (EP) 1, 2 and where relevant 3 and 4.
-
aSNP position is based on the 2882 bp concatenated sequence.
-
bNote that SNP 416 had two extension primers to accommodate a known proximal SNP variation.
