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Table 1 Primers for primary PCR reaction for the iPLEX20SNP

From: A comparison of two informative SNP-based strategies for typing Pseudomonas aeruginosa isolates from patients with cystic fibrosis

SNP site Gene target Primers (5′-3′)
7 and 45 acsA P1 10mer-ACCTTGTGCTTGTCGATGAT
P2 10mer-GCCACACCTACATCGTCTAT
322, 381 and 387 acsA P1 10mer-ATCAGGTTGCCGAGGTTGTC
P2 10mer-AGACCGGCGCCTGCCTGATG
416 and 488 aroE P1 10mer-TCGGTGTTGTCGCCGCGCAG
P2 10mer-CAATGTCACCGTGCCGTTCA
881 aroE P1 10mer-CAGAGGAAGAATGCCTCGG
P2 10mer-CGACATGATGTATGCCAAGG
894 and 937 guaA P1 10mer-AACATCGTCGACGACGCCAT
P2 10mer-AACACGCAGGTCAGTTGGTC
1086 and 1152 guaA P1 10mer-GGCGAGGAACTTCACGTCCTG
P2 10mer-ATGGGCGTGAAGGTGATCCG
1297 mutL P1 10mer-AGAAGACCGAGTTCGACCAT
P2 10mer-AAGATGGTCTTGCCGTTGTG
1465 mutL P1 10mer-ACCAGCTTGTCGCGCACCAT
P2 10mer-AGCGCAACGGCCTGCACCT
1865 and 1958 nuoD P1 10mer-TACAGCAGGTGGTTCAGGAT
P2 10mer-AAGATGGCCGAGCGCCAGT
2169 and 2208 ppsA P1 10mer-AGAGAAGGGGACCGTCCTG
P2 10mer-ACCTTGTCCATTTCCGACAC
2337 ppsA P1 10mer-TGGTCTCCGACATGACCGA
P2 10mer-TTCGCGAGCGATGATCGCC
2551 trpE P1 10mer-GGATCAACGAAGAGGCCGA
P2 10mer-TCGATCAGCATCAGGTGCTC
  1. P1 = forward amplification primer;
  2. P2 = reverse amplification primer;
  3. 10mer = 5′ 10-mer tagACGTTGGATG.