Table 1 Primers for primary PCR reaction for the iPLEX20SNP
SNP site | Gene target | Primers (5′-3′) |
|---|---|---|
7 and 45 | acsA | P1 10mer-ACCTTGTGCTTGTCGATGAT |
P2 10mer-GCCACACCTACATCGTCTAT | ||
322, 381 and 387 | acsA | P1 10mer-ATCAGGTTGCCGAGGTTGTC |
P2 10mer-AGACCGGCGCCTGCCTGATG | ||
416 and 488 | aroE | P1 10mer-TCGGTGTTGTCGCCGCGCAG |
P2 10mer-CAATGTCACCGTGCCGTTCA | ||
881 | aroE | P1 10mer-CAGAGGAAGAATGCCTCGG |
P2 10mer-CGACATGATGTATGCCAAGG | ||
894 and 937 | guaA | P1 10mer-AACATCGTCGACGACGCCAT |
P2 10mer-AACACGCAGGTCAGTTGGTC | ||
1086 and 1152 | guaA | P1 10mer-GGCGAGGAACTTCACGTCCTG |
P2 10mer-ATGGGCGTGAAGGTGATCCG | ||
1297 | mutL | P1 10mer-AGAAGACCGAGTTCGACCAT |
P2 10mer-AAGATGGTCTTGCCGTTGTG | ||
1465 | mutL | P1 10mer-ACCAGCTTGTCGCGCACCAT |
P2 10mer-AGCGCAACGGCCTGCACCT | ||
1865 and 1958 | nuoD | P1 10mer-TACAGCAGGTGGTTCAGGAT |
P2 10mer-AAGATGGCCGAGCGCCAGT | ||
2169 and 2208 | ppsA | P1 10mer-AGAGAAGGGGACCGTCCTG |
P2 10mer-ACCTTGTCCATTTCCGACAC | ||
2337 | ppsA | P1 10mer-TGGTCTCCGACATGACCGA |
P2 10mer-TTCGCGAGCGATGATCGCC | ||
2551 | trpE | P1 10mer-GGATCAACGAAGAGGCCGA |
P2 10mer-TCGATCAGCATCAGGTGCTC |