Skip to main content

Table 1 Validation of previously identified Chagas biomarkers within HUG study samples

From: Serum biomarkers predictive of cure in Chagas disease patients after nifurtimox treatment

m/z(/1,000) Fractions and chemistries P value (CD + vs HC) AUC for ROC curve (fold CD/HC) P value (FU vs HC) AUC for ROC curve (fold FU/HC) P value (CD + vs FU) AUC for ROC curve (fold CD/FU) Mean signal intensity ± SEM
HC CD+ FU
(n = 37) (n = 37) (n = 37)
3.7 F1 CM10 0.0001, 1.0 (+4.2) 0.0001, 0.88 (+2.3) 0.0011, 0.82 (+1.8) 1.54 ± 0.11 6.46 ± 0.64 3.60 ± 0.45
4.4 F1 IMAC; F1,5 CM10 0.0001, 1.0 (+3.7) 0.0159, 0.69 (+1.7) 0.0001, 0.91 (+2.2) 7.66 ± 0.53 28.35 ± 1.74 12.84 ± 1.91
5.4a F1, 3 CM10 0.0001, 1.0 (-0.3) 0.0001, 0.99 (-0.2) 0.2427, 0.65 (+1.2) 3.51 ± 0.31 0.90 ± 0.07 0.75 ± 0.10
6.6 F3,5 CM10 0.0001, 1.0 (+3.4) 0.0001, 0.91 (+2.5) 0.0028, 0.79 (+1.4) 3.65 ± 0.26 12.45 ± 0.62 8.96 ± 0.89
7.8 F1,3 CM10 0.0001, 0.97 (+3.9) 0.3405, 0.55 (+1.2) 0.0005, 0.91 (+3.15) 0.55 ± 0.07 2.14 ± 0.35 0.68 ± 0.12
9.3 F1,4 IMAC; F1,3 CM10 0.0001, 0.92 (+1.7) 0.9456, 0.51 (+1.0) 0.0001, 0.87 (+1.7) 2.74 ± 0.26 4.76 ± 0.24 2.77 ± 0.31
10.1 F1,3,4,6 IMAC; F1 CM10 0.0001, 0.88 (+2.0) 0.3622, 0.56 (-0.9) 0.0001, 0.96 (+2.3) 15.27 ± 1.80 30.48 ± 2.92 13.13 ± 1.42
12.7 F2,5 IMAC; F1,5,6 CM10 0.0003, 0.80 (+2.0) 0.5879, 0.53 (+1.2) 0.0129, 0.73 (+1.7) 2.59 ± 0.41 5.27 ± 0.55 3.02 ± 0.68
13.6 F1 IMAC; F1 CM10 0.0001, 0.96 (+2.3) 0.2220, 0.59 (+1.2) 0.0001, 0.86 (+1.8) 4.43 ± 0.51 10.07 ± 0.62 5.50 ± 0.71
15.2 F4,5 IMAC; F1,3,6 CM10 0.0001, 1.0 (+2.9) 0.6538, 0.51 (+1.1) 0.0001, 0.96 (+2.7) 5.62 ± 0.51 16.46 ± 1.33 6.04 ± 0.75
16.3 F4 IMAC; F3,6 CM10 0.0001, 0.95 (+3.1) 0.4349, 0.58 (+1.2) 0.0001, 0.94 (+2.6) 1.77 ± 0.30 5.48 ± 0.48 2.10 ± 0.28
24.7 F2 IMAC 0.0001, 0.84 (+1.8) 0.6429, 0.51 (-0.9) 0.0001, 0.85 (+1.9) 1.48 ± 0.16 2.61 ± 0.19 1.37 ± 0.18
28.1a F1,3 CM10 0.0001, 0.96 (-0.8) 0.3598, 0.56 (-1.0) 0.0032, 0.79 (-0.8) 28.43 ± 0.63 21.66 ± 1.07 27.06 ± 1.23
28.9 F1 IMAC 0.0001, 0.98 (+3.5) 0.0182, 0.66 (+1.9) 0.0006, 0.80 (+1.9) 0.46 ± 0.07 1.61 ± 0.13 0.86 ± 0.15
75.4 F1 CM10 0.0001, 1.0 (+3.0) 0.0005, 0.83 (+1.9) 0.0001, 0.96 (+1.6) 1.18 ± 0.15 3.53 ± 0.09 2.25 ± 0.23
  1. a Downregulated protein in CD-positive subjects.
  2. Mass (m/z), mean intensities, and AUC’s for selected differentially expressed peptides/proteins between HC, 2008-CD + and 2011-FU sera samples.
  3. An area under the curve (AUC) of 1 suggests good diagnostic power, while AUC values that approach 0.5 indicate little diagnostic potential. HC: Healthy control; CD: Chagas disease; FU: follow-up.