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Figure 3 | BMC Infectious Diseases

Figure 3

From: Serum biomarkers predictive of cure in Chagas disease patients after nifurtimox treatment

Figure 3

Immunoblot analysis of ZOOM fractions using specific anti-neoepitope antisera and Coomassie gel used for in-gel trypsin digestion. Coomassie, Ponceau Red and Immunoblot analysis of pooled sera fractionated by ZOOM IEF Fractionation validate SELDI results. (A) ZOOM fractions 2 and 3 probed with anti-APOA1(24) rabbit serum confirms the presence and up-regulation of the 24.7 kDa APOA1 biomarker in 2008-CD + samples vs healthy control (HC) samples especially in fraction 3 (pH range 5.4-7). (B) ZOOM fractions 1 and 2 probed with anti-FN1(28) confirms the presence and up-regulation of the 28.9 kDa FN1 biomarker in 2008-CD + vs HC samples in fraction 2 (pH range 7-9.1).

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