Table 1 Primers used
From: Distribution of superantigens in group A streptococcal isolates from Salvador, Brazil
Gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Annealing temp. °C | Product size (bp) |
|---|---|---|---|---|
speA1-3,6 [15] | 5′-GGACTAACAATCTCGCAAGAGG-3′ | 5′-TTACTTGGTTGTTAGGTAGACTTC-3′ | 54 | 696 |
speA4,5 [15] | 5′-GCTAACAACCTCACAAGAAG -3′ | 5′-TGCTTGAGTCAAGCGTCTC-3′ | 53 | 659 |
speC [15] | 5′-GATTTCTACTATTTCACC-3′ | 5′-AAATATCTGATCTAGTCCC-3′ | 44 | 584 |
speG [15] | 5′-CTTATGCAGATGAAAATTTAAAAGATT-3′ | 5′-AAAGCAAAGGGGGGAGAATAG-3′ | 53.2 | 664 |
speI [15] | 5′-ATGAGTAGTGTGGGAGTTATTAA-3′ | 5′-ATGAAGTTGATCAGAATAAGCG-3′ | 55 | 645 |
speJ [15] | 5′-ATCTTATTTAGTCCAAAGGTAAAT-3′ | 5′-GTGAACGAGGAGAGGTATGAA-3′ | 55.5 | 718 |
smeZ [15] | 5′-TTGGATTAGAAGTAGATAA-3′ | 5′-TTGAATTAGGAGTCAATTTCTATATCT-3′ | 52 | 638 |
ssa [15] | 5′-AGTCAGCCTGACCCTACTCCA-3′ | 5′-TAAGGTGAACCTCTATAGCTATAG-3′ | 59.1 | 691 |
speH [6] | 5′-TTGGATCCAATTCTTATAATACAACC-3′ | 5′-CCACTTCCTGAGCGGTTACTTTCGG-3′ | 50 | 399 |
speJ [6] | 5′-CTATGGTGGAATTACACC-3′ | 5′-CATGTTTATTGCCATTGATCGC-3′ | 50 | 247 |
speK [6] | 5′-GCGGATCCGATACGTACAATACAAATG-3′ | 5′-GCGAATTCAATAGCATTCAACCA-3′ | 50 | 798 |
speL [6] | 5′-GCAGCCATATGGAAGAGACTATTCCTCTTAAGGATATATACTCTC-3′ | 5′-GGGGATCCTTAATTTTCTTTGTTTGTGAATAAATAGAC-3′ | 50 | 703 |
speM [6] | 5′-GCAGCCATATGTTTTCAGATGCTGTGTTGG-3′ | 5′-GGGGATCCTTAATTTTTAGAAAAATCTTCGTTTAAGTA-3′ | 50 | 661 |
speB [6] | 5′-GGATCCCAACCAGTTGTTAAATCTCT-3′ | 5′-AACGTTCTAAGGTTTGATGCCTACAA-3′ | 50 | 774 |