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Table 1 Primers used

From: Distribution of superantigens in group A streptococcal isolates from Salvador, Brazil

Gene Forward primer (5′ to 3′) Reverse primer (5′ to 3′) Annealing temp. °C Product size (bp)
speA1-3,6 [15] 5′-GGACTAACAATCTCGCAAGAGG-3′ 5′-TTACTTGGTTGTTAGGTAGACTTC-3′ 54 696
speA4,5 [15] 5′-GCTAACAACCTCACAAGAAG -3′ 5′-TGCTTGAGTCAAGCGTCTC-3′ 53 659
speC [15] 5′-GATTTCTACTATTTCACC-3′ 5′-AAATATCTGATCTAGTCCC-3′ 44 584
speG [15] 5′-CTTATGCAGATGAAAATTTAAAAGATT-3′ 5′-AAAGCAAAGGGGGGAGAATAG-3′ 53.2 664
speI [15] 5′-ATGAGTAGTGTGGGAGTTATTAA-3′ 5′-ATGAAGTTGATCAGAATAAGCG-3′ 55 645
speJ [15] 5′-ATCTTATTTAGTCCAAAGGTAAAT-3′ 5′-GTGAACGAGGAGAGGTATGAA-3′ 55.5 718
smeZ [15] 5′-TTGGATTAGAAGTAGATAA-3′ 5′-TTGAATTAGGAGTCAATTTCTATATCT-3′ 52 638
ssa [15] 5′-AGTCAGCCTGACCCTACTCCA-3′ 5′-TAAGGTGAACCTCTATAGCTATAG-3′ 59.1 691
speH [6] 5′-TTGGATCCAATTCTTATAATACAACC-3′ 5′-CCACTTCCTGAGCGGTTACTTTCGG-3′ 50 399
speJ [6] 5′-CTATGGTGGAATTACACC-3′ 5′-CATGTTTATTGCCATTGATCGC-3′ 50 247
speK [6] 5′-GCGGATCCGATACGTACAATACAAATG-3′ 5′-GCGAATTCAATAGCATTCAACCA-3′ 50 798
speL [6] 5′-GCAGCCATATGGAAGAGACTATTCCTCTTAAGGATATATACTCTC-3′ 5′-GGGGATCCTTAATTTTCTTTGTTTGTGAATAAATAGAC-3′ 50 703
speM [6] 5′-GCAGCCATATGTTTTCAGATGCTGTGTTGG-3′ 5′-GGGGATCCTTAATTTTTAGAAAAATCTTCGTTTAAGTA-3′ 50 661
speB [6] 5′-GGATCCCAACCAGTTGTTAAATCTCT-3′ 5′-AACGTTCTAAGGTTTGATGCCTACAA-3′ 50 774
  1. All primer sequences shown here have previously been published by Maripuu et al. or Commons et al.