Table 1 Multiplex PCR on protozoa and nematodes
Name | Sequence (label & quencher) | Amount | Target organism |
|---|---|---|---|
Multiplex PCR on protozoa | |||
Crypto-tp | Tex Red-ccaatcacagaatcatcagaatcgactggtatc-BHQ2 | 5 pmol | Cryptosporidium sp. |
Crypto CrF | cgcttctctagccttttcatga | 15 pmol | |
Crypto CrR | cttcacgtgtgtttgccaat | 15 pmol | |
Giardia-tp | Fam-cccgcggcggtccctgctag-BHQ1 | 1 pmol | G. lamblia |
Giardia 80 F | gacggctcaggacaacggtt | 5 pmol | |
Giardia 127R | ttgccagcggtgtccg | 5 pmol | |
Df-172 | Vic-caattctagccgcttat-BHQ1 | 5 pmol | D. fragilis |
Df 124-F | caacggatgtcttggctcttta | 14 pmol | |
Df 221-R | tgcattcaaagatcgaacttatcac | 14 pmol | |
PhHV TP | Cy5-tttttatgtgtccgccaccatctggatc-BHQ2 | 7.5 pmol | Phocine Herpes virus |
PhHV-F | gggcgaatcacagattgaatc | 10 pmol | |
PhHV-R | gcggttccaaacgtaccaa | 10 pmol | |
Multiplex PCR on nematodes | |||
Ad155MGB-TP | Fam-atcgtttaccgactttag-BHQ1MGB | 2.5 pmol | A. duodenale |
Ad125F | gaatgacagcaaactcgttgttg | 5 pmol | |
Ad195R | atactagccactgccgaaacgt | 5 pmol | |
Na-TP | Tex Red-gtgttcagcaattcccgtttaagtgaag-BHQ2 | 1.25 pmol | N. americanus |
Na58F | ctgtttgtcgaacggtacttgc | 5 pmol | |
Na158R | ataacagcgtgcacatgttgc | 5 pmol | |
Alum124T-TP | Vic-ttggcggacaattgcatgcgat-BHQ1 | 1.25 pmol | Ascaris sp. |
Alum96F | gtaatagcagtcggcggtttctt | 2 pmol | |
Alum183R | gcccaacatgccacctattc | 2 pmol | |
Stro TP | ATTO425-acacaccggccgtcgctgc-BHQ1 | 1.25 pmol | S. stercoralis |
Stro18s-1530F | gaattccaagtaaacgtaagtcattagc | 2.5 pmol | |
Stro18s-1630R | tgcctctggatattgctcagttc | 2.5 pmol | |