in BALB/c mice that were infected by intraperitoneal injection of 10
6 CFU (low dose) or 10
7 CFU per mouse (high dose) of
strain LNP 24198lux expressing the luciferase. Mice were analyzed for bioluminescence at the indicated times. Images depict photographs overlaid with colour representations of luminescence intensity, measured in photons/s-cm2-sr and indicated on the scales, where red is most intense and blue is least intense. (Top row) (A) Dorsal views of 8 mice (high dose) and 7 mice (low dose). A non infected mouse (NI) is also shown (B). The luminescence was quantified and expressed as means ± SD from each category at the indicated times by defining specific representative region of interest encompassing the entire animal. (C) Western blotting of CSF and blood from mice to detect lipocalin 2 (arrows) after the indicated time points of infection and using 106 CFU (low dose) or 107 CFU per mouse (high dose) as in (A).