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Table 1 Multi-centre comparison of the PCR assay for detection of carbapenemases, and the PCR platforms, and DNA polymerases used

From: Multi-centre evaluation of real-time multiplex PCR for detection of carbapenemase genes OXA-48, VIM, IMP, NDM and KPC

Strain ID Laboratory1 Laboratory2 Laboratory31 Laboratory41 Laboratory52 Laboratory62 Laboratory72
OXA 8-90 OXA-48 OXA-48 OXA-48 OXA-48 OXA-48 OXA-48 OXA-48
OXA 8-17 OXA-48/CTX-M OXA-48/CTX-M OXA-48/CTX-M OXA-48/CTX-M OXA-48 OXA-48 OXA-48
S 4-2 OXA-48/CTX-M OXA-48/CTX-M OXA-48/CTX-M OXA-48/CTX-M OXA-48 OXA-48 OXA-48
S3-60 OXA-48/CTX-M OXA-48/CTX-M OXA-48/CTX-M OXA-48/CTX-M OXA-48 OXA-48 OXA-48
GR-21/PM-302 VIM VIM VIM VIM VIM VIM VIM
GR-04/KP-69 VIM/KPC VIM/KPC VIM/KPC VIM/KPC VIM/KPC VIM/KPC VIM/KPC
GR-38/KP-139 VIM VIM VIM VIM VIM VIM VIM
GR-31/KP-956 VIM/KPC VIM/KPC VIM/KPC VIM/KPC VIM/KPC VIM/KPC VIM/KPC
GR-23/KP-385 KPC KPC KPC KPC KPC KPC KPC/imp*
New York-11 KPC KPC KPC KPC KPC KPC KPC
New York-3 KPC KPC KPC KPC KPC KPC KPC
JS022 NDM/CTX-M NDM/CTX-M NDM/CTX-M NDM/CTX-M NDM NDM NDM
RC-89 NDM/CTX-M NDM/CTX-M NDM/CTX-M NDM/CTX-M NDM NDM NDM
S 3-62 IMP IMP IMP IMP IMP IMP IMP
S5-36 IMP IMP IMP IMP IMP IMP IMP
RC-79 CTX-M CTX-M CTX-M CTX-M negative negative negative
EIE-UMC-1 CTX-M CTX-M negative negative negative negative negative
RC-8 negative negative negative negative negative negative negative
RC-16 negative negative negative negative negative negative negative
GR-07/KP-3878 negative negative negative negative negative negative negative
thermocycler Biorad CFX LC 480 ABI 7500 ABI 7500 LC 480 ABI 7500 LC 480
    Biorad CFX     
polymerase Sigma Roche ABI ABI Roche N-Amp Roche
    Biorad     
  1. 1 Only CTX-M group I PCR was performed.
  2. 2 No CTX-M PCR was performed.
  3. *: Background IMP was detected, and scored as inconclusive by laboratory 7.