Multi-centre evaluation of real-time multiplex PCR for detection of carbapenemase genes OXA-48, VIM, IMP, NDM and KPC

Table 1 Multi-centre comparison of the PCR assay for detection of carbapenemases, and the PCR platforms, and DNA polymerases used

Strain ID	Laboratory1	Laboratory2	Laboratory3¹	Laboratory4¹	Laboratory5²	Laboratory6²	Laboratory7²
OXA 8-90	OXA-48	OXA-48	OXA-48	OXA-48	OXA-48	OXA-48	OXA-48
OXA 8-17	OXA-48/CTX-M	OXA-48/CTX-M	OXA-48/CTX-M	OXA-48/CTX-M	OXA-48	OXA-48	OXA-48
S 4-2	OXA-48/CTX-M	OXA-48/CTX-M	OXA-48/CTX-M	OXA-48/CTX-M	OXA-48	OXA-48	OXA-48
S3-60	OXA-48/CTX-M	OXA-48/CTX-M	OXA-48/CTX-M	OXA-48/CTX-M	OXA-48	OXA-48	OXA-48
GR-21/PM-302	VIM	VIM	VIM	VIM	VIM	VIM	VIM
GR-04/KP-69	VIM/KPC	VIM/KPC	VIM/KPC	VIM/KPC	VIM/KPC	VIM/KPC	VIM/KPC
GR-38/KP-139	VIM	VIM	VIM	VIM	VIM	VIM	VIM
GR-31/KP-956	VIM/KPC	VIM/KPC	VIM/KPC	VIM/KPC	VIM/KPC	VIM/KPC	VIM/KPC
GR-23/KP-385	KPC	KPC	KPC	KPC	KPC	KPC	KPC/imp*
New York-11	KPC	KPC	KPC	KPC	KPC	KPC	KPC
New York-3	KPC	KPC	KPC	KPC	KPC	KPC	KPC
JS022	NDM/CTX-M	NDM/CTX-M	NDM/CTX-M	NDM/CTX-M	NDM	NDM	NDM
RC-89	NDM/CTX-M	NDM/CTX-M	NDM/CTX-M	NDM/CTX-M	NDM	NDM	NDM
S 3-62	IMP	IMP	IMP	IMP	IMP	IMP	IMP
S5-36	IMP	IMP	IMP	IMP	IMP	IMP	IMP
RC-79	CTX-M	CTX-M	CTX-M	CTX-M	negative	negative	negative
EIE-UMC-1	CTX-M	CTX-M	negative	negative	negative	negative	negative
RC-8	negative	negative	negative	negative	negative	negative	negative
RC-16	negative	negative	negative	negative	negative	negative	negative
GR-07/KP-3878	negative	negative	negative	negative	negative	negative	negative
thermocycler	Biorad CFX	LC 480	ABI 7500	ABI 7500	LC 480	ABI 7500	LC 480
			Biorad CFX
polymerase	Sigma	Roche	ABI	ABI	Roche	N-Amp	Roche
			Biorad

ISSN: 1471-2334