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Table 3 Parasites detected by different diagnostic methods in the samples of the 1,477 infected patients

From: Intestinal parasitoses in a tertiary-care hospital located in a non-endemic setting during 2006–2010

Parasite No. of parasites Diagnostic methods
Maonly Cbonly Both M and Cb PCRc Both M and PCRc Both C and PCRc M, C, and PCRc Both IC and Ma Both IC and IFa
Protozoa 1,789          
Blastocystis hominis 1,234 502 (5.64%) 55 (6.07%) 677 (74.2%) - - - - - -
Giardia intestinalis 168 0 - - - - - - 163 (1.83%) 5 (0.06%)
Dientamoeba fragilis 149 41 (0.46%) 3 (0.33%) 0 60 (12.22%) 3 (0.61%) 39 (7.94%) 3 (0.61%) - -
Entamoeba coli 148 110 (1.24%) 0 38 (4.19%) - - - - - -
Entamoeba dispar 69 - - - 7 (0.77%) 26 (2.87%) 5 (0.55%) 31 (3.42%) - -
Cryptosporidium spp. 17 - - - - - - - - 17 (0.19%)
Entamoeba histolytica 4 - - - 3 (0.33%) 1 (0.11%) 0 0 - -
   M a,e only C b,f only Both M and C b,f ST d only Both M and ST d,e MA a only Both M and MA a,g   
Helminths 126          
Strongyloides stercoralis 36 - 17 (1.88%) 29 (0.33%) - - - -   
Enterobius vermicularis 35 27 (0.3%) - - 6 (5.17%) 2 (1.72%) - -   
Hymenolepis nana 12 12 (0.13%) - - - - - -   
Taenia saginata 12 0 - - - - 3 (0.13%) 9 (0.10%)   
Taenia spp. 9 7 (0.08%) - - - - 0 2 (0.02%)   
Ascaris lumbricoides 9 7 (0.08%) - - - - 1 (0.01%) 1 (0.01%)   
Trichuris trichiura 6 6 (0.07%) - - - - 0 0   
Ancylostoma duodenale 4 0 4 (0.44%) - - - - -   
Dicrocoelium dendriticum 2 2 (0.02%) - - - - - -   
Schistosoma mansoni 1 1 (0.01%) - - - - - -   
Total 1,915          
  1. M: Microscopic examination; C: Culture; IC: Immunocromatographic assay for the detection of specific antigens of Cryptosporidium parvum and Giardia intestinalis; IF: Immunofluorescence assay to detect Cryptosporidium spp. oocysts and G. intestinalis cysts; ST: Scotch Test; MA: Macroscopic examination; − : Method not applicable.
  2. a: in brackets the % on 8,886 patients are reported.
  3. b: in brackets the % on 906 patients are reported.
  4. c: in brackets the % on 906 and on 491 patients are reported, when PCR assays for the differentiation of E. histolytica and E. dispar and the real-time PCR assay for the identification of D. fragilis were respectively applied.
  5. d: in brackets the % on 116 patients are reported.
  6. e: microscopic examination is referred to ova identification.
  7. f: microscopic examination and culture are referred to larvae identification.
  8. g: microscopic examination is referred to ova identification and macroscopic identification of adult stages.