DNA-Sm14/DNA-Hsp65 immunization reduced tissue damage during granuloma formation in the liver of
infected mice. Representative photomicrography of HE, Picrosirius and α-SMA staining from liver sections of mice without immunization (Control), immunized with DNA-Sm14 or DNA-Sm14/DNa-Hsp65, 69 days after infection. HE and Picrosirius staining were used to analyze granuloma formation and collagen deposition respectively. Immunohistochemical staining was performed to detect α-SMA fibers around the granuloma. Original magnification, X50.