Simultaneous vaccination with DNA-Sm14/DNA-Hsp65 reduces egg viability. Two weeks after the last immunization, the mice were infected with 30 cercariae, and the developmental stages of the eggs were evaluated in the intestinal tissue 48 days post infection. The intestinal eggs were identified as immature, mature or dead using the Oogram technique. The results are expressed as the mean ± standard error from 6-7 animals/group and are representative of two experiments. *p < 0.05 vs Control group.