A combined vaccination strategy using DNA-Sm14/DNA-Hsp65 increased the number of CD8+ memory T cells in the spleen of infected mice. A) The number of CD8+ CD44hiCD62Llow memory T lymphocytes in the spleen of mice without immunization (Control) or immunized with DNA-Sm14 or DNA-Sm14/DNA-Hsp65 and infected with S. mansoni over time (15, 48 and 105 days after infection). B) The ratio of CD8+ memory T lymphocytes / total number of CD8+ in the spleen of mice vaccinated with DNA-Sm14 or DNA-Sm14/DNA-Hsp65 and infected with S. mansoni on days 15 and 48 post infection. After immunization, the mice were infected with 30 cercariae, and the phenotype of the memory T lymphocytes in the spleen was evaluated using flow cytometry. The results are expressed as the mean ± standard error from 3-5 animals/group and are representative of two experiments. A) *p < 0.05 DNA-Sm14/DNA-Hsp65 48 days vs 15 days; DNA-Sm14 48 days vs 15 days; #p < 0.05 DNA-Sm14/DNA-Hsp65 69 days vs 48 days; DNA-Sm14 69 days vs 48 days. B) *p < 0.05 DNA-Sm14/DNA-Hsp65 48 days vs Control group 48 days.