Figure 2

dectin-1 and TLR2 participate in Mtb- induced ROS and apoptosis in PMN. A) PMN were pretreated with DHR and incubated with or without neutralizing anti-TLR2, anti-dectin-1, anti-CD11b, irrelevant IgG antibodies or the β-glucan laminarin, and then stimulated with Ra or M for 90 min at 1:2 ratio Mtb:PMN. Thereafter, the emission of oxidized DHR was evaluated by flow cytometry. Results are expressed as mean ± SE (n = 12); control (−−) vs. Ra, Ra + IgG and Ra + anti-CD11b: ***p < 0.001 (B) Apoptosis was measured in 18-hr cultured PMN with or without clinical strains, under the same conditions described in (A) and evaluated by flow cytometry. Results are expressed as the percentage of AV-FITC positive cells (% PMN AV + ± SE) (n = 20); control (−−) vs. Ra, Ra + IgG and Ra + anti-CD11b: ***p < 0.001. (C) TLR2 and (D) dectin-1 expression was measured at the surface of the PMN after 30, 60 or 180 minutes culture, in the presence of Mtb (1:2 Mtb:PMN ratio) (n = 16) and evaluated by flow cytometry.