Prevalence and genetic characterization of Cryptosporidium, Enterocytozoon, Giardia and Cyclospora in diarrheal outpatients in china

Table 1 Primers used for protozoan gene amplification

Genus	Gene	Sequence of primers (5´ → 3´)	Amplicon size (bp)
Cryptosporidium	SSUrRNA	CryF1: TTCTAGAGCTAATACATGCG	~1325
		CryR1: CCATTTCCTTCGAAACAGGA	~1325
		CryF2: GGAAGGGTTGTATTTATTAGATAAAG	~840
		CryR2: CTCATAAGGTGCTGAAGGAGTA	~840
Microsporidia	SSUrRNA	EF1: GATGGTCATAGGGATGAAGAGCTT	~1200
		ER1: AATACAGGATCACTTGGATCCGT	~1200
		EF2: AGGGATGAAGAGCTTCGGCTCTG	~600
		ER2: AATATCCCTAATACAGGATCACT	~600
Giardia	TPI	TPIF1: AAATIATGCCTGCTCGTCG	~605
		TPIR1: CAAACCTTITCCGCAAACC	~605
		TPIF2: CCCTTCATCGGIGGTAACTT	~530
		TPIR2: GTGGCCACCACICCCGTGCC	~530
Cyclospora	18SrRNA	CYCLF1: AATGTAAAACCCTTCCAGAGTAAC	~1000
		CYCLR1: GCAATA ATCTATCCCCATCACG	~1000
		CYCLF2: AATTCCAGCTCCAATAGTGTAT	~500
		CYCLR2: CAGGAGAAGCCAAGGTAGGCRTTT

ISSN: 1471-2334