Figure 1

Mycolactone concentration in punch biopsies of untreated M. ulcerans disease lesions before antibiotic treatment. (A) Mycolactone concentration in ASL measured by cytotoxicity assay using synthetic mycolactone A/B generated calibration curves. Horizontal lines represent medians and each dot represents one lesion. (B) Mycolactone concentration in punch biopsies of untreated M. ulcerans disease lesions before antibiotic treatment. Mycolactone A/B concentration in ASL measured by multiple reaction monitoring (MRM) methodology with liquid chromatographic separation coupled to a tandem mass spectrometer. Horizontal lines represent medians and each dot represents one lesion.