BMC Infectious Diseases

Table 4 Mutations of the katG, embB, rrs, gyrA and rpsl genes identified by pyrosequencing in clinical isolates and sputum samples

From: Pyrosequencing for rapid detection of Tuberculosis resistance in clinical isolates and Sputum samples from re-treatment Pulmonary Tuberculosis patients

Drugs	Genes	Amino acid change(s)	Codon change(s)	Nucleotide change	BACTEC 960 tests
Drugs	Genes	Amino acid change(s)	Codon change(s)	Nucleotide change	R	S
INH	katG
		S315T	AGC/ACC		133
		S315N	AGC/AAC		5
		S315T	AGC/ACA		3
		WT			46	39
		Fail			3
SM	rpsl
		K43R	AAG/AGG		100	2
		K88R	AAG/AGG		18	1
		WT			32	71
		Fail			3	2
EMB	embB
		M306I	ATG/ATA		17	4
		M306I	ATG/ATC		2	2
		M306I	ATG/CTG		7	2
		M306V	ATG/GTG		50	4
		WT			57	80
		Fail			4
OFL	gyrA
		G88A	GGC/GCC		1
		G88C	GGC/TGC		2
		A90V	GCG/GTG		22
		S91P	TCG/CCG		8	1
		D94N	GAC/AAC		5
		D94H	GAC/CAC		3
		D94A	GAC/GCC		18
		D94G	GAC/GGC		54
		D94Y	GAC/TAC		5
		A90V + D94N	GCG/GTG + GAC/AAC		1
		A90V + D94G	GCG/GTG + GAC/GGC		1
		A90V + D94Y	GCG/GTG + GAC/TAC		1
		WT			19	86
		Fail			2
AMK	rrs
				A1401G	34
				WT	10	178
		Fail				7

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ISSN: 1471-2334

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