Immunogen | FI-MCMV | ELISA titer (2n) of IgG antibodyb | Protection against SG-MCMV challenge | ||
---|---|---|---|---|---|
Dosage (μg) | Immunization once | Immunization twice | Spleen virus titers (log10PFU/ml) | Survival mice/tested mice | |
Adjuvant-free | 0.25 | 3.67 ± 1.15 | 5.33 ± 0.58 | 5.27 ± 0.19 | 0/6 |
+ Chitosan | 5.00 ± 1.00 | 9.67 ± 1.15c | 5.08 ± 0.13 | 0/6 | |
+ Alum | 6.33 ± 1.53c | 11.33 ± 1.15c | 5.14 ± 0.11 | 0/6 | |
+ MF59 | 7.33 ± 0.58c | 12.33 ± 1.53c | 4.58 ± 0.36c,e | 2/6 | |
Adjuvant-free | 1 | 4.67 ± 1.15 | 10.00 ± 1.73 | 5.13 ± 0.29 | 0/6 |
+ Chitosan | 6.33 ± 0.58 | 12.00 ± 1.00c | 4.64 ± 0.50e | 2/6 | |
+ Alum | 7.33 ± 0.58c | 12.33 ± 1.15c | 4.78 ± 0.15e | 1/6 | |
+ MF59 | 10.67 ± 1.15c,d | 15.00 ± 1.00c,d | 3.51 ± 0.49c,d,e | 5/6e | |
Adjuvant-free | 4 | 7.67 ± 0.58 | 11.67 ± 1.52 | 4.41 ± 0.48e | 3/6 |
+ Chitosan | 10.00 ± 1.73c | 13.67 ± 0.58c | 4.02 ± 0.51e | 5/6e | |
+ Alum | 10.33 ± 0.58c | 14.00 ± 1.00c | 3.80 ± 0.11c,e | 6/6e | |
+ MF59 | 11.67 ± 0.58c | 16.33 ± 0.58c,d | 2.56 ± 0.77c,d,e | 6/6e | |
Chitosan only | - | Undetected | Undetected | 5.87 ± 0.17 | 0/6 |
Alum only | Undetected | Undetected | 5.68 ± 0.27 | 0/6 | |
MF59 only | Undetected | Undetected | 5.62 ± 0.38 | 0/6 | |
Mock | Undetected | Undetected | 5.73 ± 0.34 | 0/6 | |
PBS | - | - | 5.91 ± 0.39 | 0/6 |