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Table 1 Reactivity of the rPPDK and rGal/GalNAc lectin from blots probed with anti-human IgG-HRP, IgG 1 -HRP and IgG 4 -HRP

From: RETRACTED ARTICLE: Production of recombinant Entamoeba histolyticapyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess

Recombinant proteins

Secondary conjugate peroxidase

Serum from patients with ALA (Group A)

Serum from healthy individuals and patients with other diseases

Pyruvate phosphate dikinase (rPPDK)

IgG

86.7% (13/15)

97.5% (39/40)

IgG1

80.0% (12/15)

97.5% (39/40)

IgG4

93.3% (14/15)

100% (40/40)

Gal/GalNAc lectin

IgG

86.7% (13/15)

97.5% (39/40)

(rGal/GalNAc lectin)

IgG1

80.0% (12/15)

87.5% (35/40)

 

IgG4

93.3% (14/15)

95.0% (38/40)