Figure 2

SDS-PAGE analysis shows the purified rGal/GalNAc lectin. Lane 1: Precision Plus Protein™ Unstained Standard Marker (Bio-Rad, USA); Lane 2: Non-induced cell from pET28a/Gal-GalNAc lectin; Lane 3: Induced cell from pET28a/Gal-GalNAc lectin; Lanes 4–5: purified rGal/GalNAc lectin protein from elution buffer (250 mM imidazole). Arrow indicates the recombinant Gal/GalNAc lectin protein (~53 kDa).