Activation of naïve CD4
T cells by BCG-dHCM through activation of DC. a. IFN-γ production from naïve CD4+ T cells by stimulation with BCG-dHCM. Monocyte-derived DC were infected with either BCG-261H or BCG-dHCM at the indicated MOI, and were used as a stimulator. 105 responder naïve CD4+ T cells were stimulated with the rBCG-infected DC at T: DC = 10: 1. b. Inhibition of T cell activation by the treatment of BCG-dHCM-infected DC with mAb. DC were infected with BCG-dHCM at the indicated MOI, and subsequently treated with 10 μg/ml of the mAb or normal murine IgG. These APCs were used as the stimulator of the indicated responder T cells (1 × 105/well) at the indicated T/DC ratios for 4 days. IFN-γ produced by T cells was measured. c. Effect of chloroquine treatment of DC on the activation of T cells. Immature DC were treated with chloroquine (50 μM, 2 h) or untreated, and subsequently infected with BCG-dHCM at the indicated MOI. These DC were used as the stimulator of the indicated responder T cells at the indicated responder/stimulator ratios. IFN-γ produced by T cells was measured. A representative of three separate experiments conducted by using 3 different PBMCs-donors is shown. Assays were performed in triplicate and the results are expressed as the mean ± SD. Titers were statistically compared using Student’s t-test.