Rapid PCR/ESI-MS-based molecular genotyping of Staphylococcus aureusfrom nasal swabs of emergency department patients

Kecojevic, Aleksandar; Ranken, Ray; Ecker, David J; Massire, Christian; Sampath, Rangarajan; Blyn, Lawrence B; Hsieh, Yu-Hsiang; Rothman, Richard E; Gaydos, Charlotte A

doi:10.1186/1471-2334-14-16

Table 1 Primer pairs used in the PCR/ESI-MS MRSA typing and characterization assay

From: Rapid PCR/ESI-MS-based molecular genotyping of Staphylococcus aureusfrom nasal swabs of emergency department patients

Row^a	Primer pair code	Target(s)	Accession number and coordinates	Forward and reverse primer sequences	Reference
A	BCT879	mecA	Y14051	5′-TCAGGTACTGCTATCCACCCTCAA-3′	[14]
	BCT879	mecA	4507..4581	5′-TGGATAGACGTCATATGAAGGTGTGCT-3′	[14]
	BCT2157	pta	NC_003923	5′-TCTTGTTTATGCTGGTAAAGCAGATGG-3′	[13]
	BCT2157	pta	629121..629229	5′-TGGTACACCTGGTTTCGTTTTGATGATTTGTA-3′	[13]
B	BCT2249	tufB	NC_002758.2	5′-TGAACGTGGTCAAATCAAAGTTGGTGAAGA-3′	[14]
	BCT2249	tufB	615038..616222	5′-TGTCACCAGCTTCAGCGTAGTCTAATAA-3′	[14]
	BCT2163	yqi	NC_003923	5′-TGAATTGCTGCTATGAAAGGTGGCTT-3′	[13]
	BCT2163	yqi	379057..379199	5′-TCGCCAGCTAGCACGATGTCATTTTC-3′	[13]
C	BCT3016	mupA	X75439	5′-TAGATAATTGGGCTCTTTCTCGCTTAAAC-3′	[14]
	BCT3016	mupA	2482..2573	5′-TAATCTGGCTGCGGAAGTGAAAT-3′	[14]
	BCT2161	tpi	NC_003923	5′-TCCCACGAAACAGATGAAGAAATTAACAAAAAAG-3′	[13]
	BCT2161	tpi	830671..830799	5′-TGGTACAACATCGTTAGCTTTACCACTTTCACG-3′	[13]
D	BCT3106	TSST1	NC_002758.2	5′-TCGTCATCAGCTAACTCAAATACATGGA-3′	This work
	BCT3106	TSST1	519..620	5′-TCACTTTGATATGTGGATCCGTCATTCA-3′	This work
	BCT3025	arcC	NC_003923	5′-TGAATAGTGATAGAACTGTAGGCACAATCGT-3′	[13]
	BCT3025	arcC	2724791..2724920	5′-TGCGCTAATTCTTCAACTTCTTCTTTCGT-3′	[13]
E	BCT2095	PVL genes, lukE-lukD	NC_003923	5′-TGAGCTGCATCAACTGTATTGGATAG-3′	[14]
	BCT2095	PVL genes, lukE-lukD	1529595..1531285	5′-TGGAAAACTCATGAAATTAAAGTGAAAGGA-3′	[14]
	BCT2149	aroE	NC_003923	5′-TGGGGCTTTAAATATTCCAATTGAAGATTTTCA-3′	[13]
	BCT2149	aroE	1674546..1674697	5′-TACCTGCATTAATCGCTTGTTCATCAA-3′	[13]
F	BCT2256	nuc	NC_002758.2	5′-TACAAAGGTCAACCAATGACATTCAGACTA-3′	[14]
	BCT2256	nuc	894288..894974	5′-TAAATGCACTTGCTTCAGGGCCATAT-3′	[14]
	BCT2166	yqi	NC_003923	5′-TAGCTGGCGGTATGGAGAATATGTCT-3′	[13]
	BCT2166	yqi	379190..379311	5′-TCCATCTGTTAAACCATCATATACCATGCTATC-3′	[13]
G	BCT4022	PVL / lukD	NC_003923	5′-TGGTCAACAAAGCTATATCAGTGAAGTAGAAC-3′	This work
	BCT4022	PVL / lukD	1529595..1531285	5′-TGGATCATGTCCAGACATTTCACCTAATG-3′	This work
	BCT2156	gmk	NC_003923	5′-TCACCTCCAAGTTTAGATCACTTGAGAGA-3′	[13]
	BCT2156	gmk	1191206..1191337	5′-TGGGACGTAATCGTATAAATTCATCATTTC-3′	[13]
H	BCT4024	ileS	NC_002758.2	5′-TCAGTTGCTACAAGAGGAGTATCACCT-3′	This work
	BCT4024	ileS	1247700..1250453	5′-TACTCATCTTCTTACCTTCACCGTCCATA-3′	This work
	BCT2150	aroE	NC_003923	5′-TGATGGCAAGTGGATAGGGTATAATACAG-3′	[13]
	BCT2150	aroE	1674392..1674523′	5′-TAAGCAATACCTTTACTTGCACCACCTG-3′	[13]

^aRow indicates the row of a 96-well assay plate. Two primer pairs are present in each well of the assay plate.

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ISSN: 1471-2334

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