Skip to main content

Table 1 Primer pairs used in the PCR/ESI-MS MRSA typing and characterization assay

From: Rapid PCR/ESI-MS-based molecular genotyping of Staphylococcus aureusfrom nasal swabs of emergency department patients

Rowa Primer pair code Target(s) Accession number and coordinates Forward and reverse primer sequences Reference
A BCT879 mecA Y14051 5′-TCAGGTACTGCTATCCACCCTCAA-3′ [14]
4507..4581 5′-TGGATAGACGTCATATGAAGGTGTGCT-3′
BCT2157 pta NC_003923 5′-TCTTGTTTATGCTGGTAAAGCAGATGG-3′ [13]
629121..629229 5′-TGGTACACCTGGTTTCGTTTTGATGATTTGTA-3′
B BCT2249 tufB NC_002758.2 5′-TGAACGTGGTCAAATCAAAGTTGGTGAAGA-3′ [14]
615038..616222 5′-TGTCACCAGCTTCAGCGTAGTCTAATAA-3′
BCT2163 yqi NC_003923 5′-TGAATTGCTGCTATGAAAGGTGGCTT-3′ [13]
379057..379199 5′-TCGCCAGCTAGCACGATGTCATTTTC-3′
C BCT3016 mupA X75439 5′-TAGATAATTGGGCTCTTTCTCGCTTAAAC-3′ [14]
2482..2573 5′-TAATCTGGCTGCGGAAGTGAAAT-3′
BCT2161 tpi NC_003923 5′-TCCCACGAAACAGATGAAGAAATTAACAAAAAAG-3′ [13]
830671..830799 5′-TGGTACAACATCGTTAGCTTTACCACTTTCACG-3′
D BCT3106 TSST1 NC_002758.2 5′-TCGTCATCAGCTAACTCAAATACATGGA-3′ This work
519..620 5′-TCACTTTGATATGTGGATCCGTCATTCA-3′
BCT3025 arcC NC_003923 5′-TGAATAGTGATAGAACTGTAGGCACAATCGT-3′ [13]
2724791..2724920 5′-TGCGCTAATTCTTCAACTTCTTCTTTCGT-3′
E BCT2095 PVL genes, lukE-lukD NC_003923 5′-TGAGCTGCATCAACTGTATTGGATAG-3′ [14]
1529595..1531285 5′-TGGAAAACTCATGAAATTAAAGTGAAAGGA-3′
BCT2149 aroE NC_003923 5′-TGGGGCTTTAAATATTCCAATTGAAGATTTTCA-3′ [13]
1674546..1674697 5′-TACCTGCATTAATCGCTTGTTCATCAA-3′
F BCT2256 nuc NC_002758.2 5′-TACAAAGGTCAACCAATGACATTCAGACTA-3′ [14]
894288..894974 5′-TAAATGCACTTGCTTCAGGGCCATAT-3′
BCT2166 yqi NC_003923 5′-TAGCTGGCGGTATGGAGAATATGTCT-3′ [13]
379190..379311 5′-TCCATCTGTTAAACCATCATATACCATGCTATC-3′
G BCT4022 PVL / lukD NC_003923 5′-TGGTCAACAAAGCTATATCAGTGAAGTAGAAC-3′ This work
1529595..1531285 5′-TGGATCATGTCCAGACATTTCACCTAATG-3′
BCT2156 gmk NC_003923 5′-TCACCTCCAAGTTTAGATCACTTGAGAGA-3′ [13]
1191206..1191337 5′-TGGGACGTAATCGTATAAATTCATCATTTC-3′
H BCT4024 ileS NC_002758.2 5′-TCAGTTGCTACAAGAGGAGTATCACCT-3′ This work
1247700..1250453 5′-TACTCATCTTCTTACCTTCACCGTCCATA-3′
BCT2150 aroE NC_003923 5′-TGATGGCAAGTGGATAGGGTATAATACAG-3′ [13]
1674392..1674523′ 5′-TAAGCAATACCTTTACTTGCACCACCTG-3′
  1. aRow indicates the row of a 96-well assay plate. Two primer pairs are present in each well of the assay plate.