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Table 6 Positive predictive values of Crossing point (Cp) values with different cut-off limits for detection of severe sepsis/septic shock, for blood samples with a positive SeptiFast test for Gram-positive bacteria

From: Quantitative data from the SeptiFast real-time PCR is associated with disease severity in patients with sepsis

Cut-off Cp value

Staphylococcus aureus(n = 24)

Streptococcus species(n = 13)

Gram-positive bacteria, other* (n = 5)

Gram-positive bacteria total (n = 42)

<15.0

60% (3/5)

100% (1/1)

50% (1/2)

62% (5/8)

<17.5

62% (5/8)

71% (5/7)

67% (2/3)

67% (12/18)

<20.0

55% (6/11)

38% (5/13)

80% (4/5)

52% (15/29)

<22.5

33% (8/24)

  

41% (17/42)

  1. The Septifast method has a Cp cut-off at 20 cycles for test positivity for Streptococcus species, and at 25 cycles for other Gram-positive bacteria.
  2. Data is presented as percentages (No. of SeptiFast positive samples from patients with severe sepsis /No. of SeptiFast positive samples in total, according to cut-off).
  3. *Including: Enterococcus faecalis (n = 3) and Streptococcus pneumonia (n = 2).