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Table 3 Assay performance

From: Multiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei

Organism a Target Efficiency (%) Linear range (fg/reaction) Repeatability (SD of C q ) b LOD gDNA (fg/reaction) c
B. mallei BuMC 99,0 1.4·10-1 – 1.4·106 0,072 0.2
  mau 98,7 1.4·100 – 1.4·106 0,080 4.5
B. pseudomallei BuMC 96,6 1·100 - 1·107 0,085 3,7
  psu 99,2 1·101 – 1·107 0,077 57
  1. a Organism from which genomic DNA was used for assessment of assay performance.
  2. b Values represent the average from the standard deviations calculated at 6 different dilutions from 4 replicate Cq measurements.
  3. c Values displayed represent the lowest DNA concentration at which 95% of the positive samples are detected, as calculated by using probit analysis.