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Table 3 Assay performance

From: Multiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei

Organism a

Target

Efficiency (%)

Linear range (fg/reaction)

Repeatability (SD of C q ) b

LOD gDNA (fg/reaction) c

B. mallei

BuMC

99,0

1.4·10-1 – 1.4·106

0,072

0.2

 

mau

98,7

1.4·100 – 1.4·106

0,080

4.5

B. pseudomallei

BuMC

96,6

1·100 - 1·107

0,085

3,7

 

psu

99,2

1·101 – 1·107

0,077

57

  1. a Organism from which genomic DNA was used for assessment of assay performance.
  2. b Values represent the average from the standard deviations calculated at 6 different dilutions from 4 replicate Cq measurements.
  3. c Values displayed represent the lowest DNA concentration at which 95% of the positive samples are detected, as calculated by using probit analysis.