Table 1 Primers used for enterovirus 71 VP1 sequencing by RT-PCR
From: Prevalence of enteroviruses in children with and without hand, foot, and mouth disease in China
Primer | Sequence | Position | Usage |
|---|---|---|---|
VP1-2382 F1 | 5′-ATAATAGCACTAGCGGCAGCCCA-3′ | 2382 | Nested RT-PCR, 1st round |
VP1-2415 F2 | 5′-ACCATGAAGTTGTGCAAGGA-3′ | 2415 | Nested RT-PCR, 2st round |
VP1-3387R2 | 5′-GCCCCAGACTGTTGTCCAAA-3′ | 3387 | Nested RT-PCR, 2st round |
VP1-3478R1 | 5′-GTCGCGAGAGCTGTCTTCCCA-3′ | 3478 | Nested RT-PCR, 1st round |
2833F | 5′-GAGYTRTTCACCTACATGCG-3′ | 2833 | Sequencing |
3065R | 5′-CTCGCRGGTGACATGAAYGG-3′ | 3065 | Sequencing |