Figure 1

Frequencies of cells producing effector molecules measured by ELISPOT. GrB (A-B), Prf (C-D) and IFN-γ- producing cells (E-F) were stimulated with PPD (A,C,E), rCFP-10 (B,D), and the CFP-10 peptide pool (F). Horizontal bars indicate the medians of the responses. Upper dashed lines represent the cut-offs for the positive IFN-γ results, and lower dashed lines cut-offs for the negative results (E-F). TB, n = 17 (rCFP-Prf n = 13), LTBI, n = 9 (rCFP-Prf n = 7), Surg., n = 6 (rCFP-Prf n = 4), Part., 23 (rCFP-Prf n = 21), Mod., n = 7 (rCFP-GrB n = 6), (CFP-peptides-IFN-γ, PPD-Prf, PPD-IFN-γ n = 5), (rCFP-perforin n = 3), BCG+, n = 6 (PPD-GrB, rCFP-GrB, PPD-Prf n = 4, rCFP-Prf n = 3).