Table 1 Oligonucleotides used for RTPCR and amplification and sequencing of penA, por and tbpB

Ochiai RTPCR forward

5′-GTTGGATGCCCGTACTGGG-3′

Ochiai RTPCR reverse

5′-ACCGATTTTGTAAGGCAGGG-3′

Ochiai RTPCR probe^a

5′-CGGCAAAGTGGATGCAACCGA-3′

Modified RTPCR forward

5′-TCAATACGCCTGCCTATGAG-3′

Modified RTPCR reverse

5′-GCACATCCAAAGTAGGATAAACG-3′

por forward

5′-CAAGAAGACCTCGGCAA-3′

por reverse

5′-CCGACAACCACTTGGT-3′

tbpB forward

5′-CGTTGTCGGCAGCGCGAAAAC-3′

tbpB reverse

5′-TTCATCGGTGCGCTCGCCTTG-3′

penA forward 1st half

5′-GCATCAGGATAATAATAACGAGAAG-3′

penA reverse 1st half

5′-TGTAAGGCAGGGTATTGAAT-3′

penA forward 2nd half

5′-GTTGGATGCCCGTACTGGG-3′

penA reverse 2nd half

5′-CAGCCAAAGGGGTTAACTTGCTGAAC-3′