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Table 1 Oligonucleotides used for RTPCR and amplification and sequencing of penA, por and tbpB

From: Neisseria gonorrhoeae and extended-spectrum cephalosporins in California: surveillance and molecular detection of mosaic penA

Primer DNA sequence
Ochiai RTPCR forward 5′-GTTGGATGCCCGTACTGGG-3′
Ochiai RTPCR reverse 5′-ACCGATTTTGTAAGGCAGGG-3′
Ochiai RTPCR probea 5′-CGGCAAAGTGGATGCAACCGA-3′
Modified RTPCR forward 5′-TCAATACGCCTGCCTATGAG-3′
Modified RTPCR reverse 5′-GCACATCCAAAGTAGGATAAACG-3′
por forward 5′-CAAGAAGACCTCGGCAA-3′
por reverse 5′-CCGACAACCACTTGGT-3′
tbpB forward 5′-CGTTGTCGGCAGCGCGAAAAC-3′
tbpB reverse 5′-TTCATCGGTGCGCTCGCCTTG-3′
penA forward 1st half 5′-GCATCAGGATAATAATAACGAGAAG-3′
penA reverse 1st half 5′-TGTAAGGCAGGGTATTGAAT-3′
penA forward 2nd half 5′-GTTGGATGCCCGTACTGGG-3′
penA reverse 2nd half 5′-CAGCCAAAGGGGTTAACTTGCTGAAC-3′
  1. aCovalently bonded to a 5-6-carboxyfluorescein-3-6-carboxytetramethylrhodamine fluorophore.