Capacity of the (CRA-1, CRA-2 and FRA-4) synthetic peptides to discriminate between serums infected by
in a ELISA. Chagasic (n = 31) and Leishmaniasis (n = 14) patient sera, and healthy individual sera (n = 11) were diluted 1:50 and evaluated in triplicates against the peptide sequences CRA-1 (AAKQKAAEAAAKQKAAEAC-biot), CRA-2 (AAKQRAAEAAAKQRAAEC-biot) and FRA-4 (ADRAFLDQKPERVPC-biot). All tests were considered significant (p < 0,001) and the cut-off values of 0.226 for CRA-1, 0.199 for CRA-2 and 0.254 for FRA-1 were calculated. Each point of the graphic represents the media of two experiments.