Dot plot representation of the delta Ct comparing the three PCR assays (P30-PCR, B1-PCR, RE-PCR) for 63 B1-PCR positive AFs. Horizontal bars represent the mean and range of the delta Ct for all the samples. The repetition of the genes B1 and RE compared to P30, and RE compared to B1, varied among the clinical variation within the fold variation indicated under each dot plot. The mean gain of detection of B1 PCR and RE PCR compared to the P30 PCR are indicated on the right. Note the fourteen AFs that were negative using P30-PCR.