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Table 2 Distribution of results with the first singlicate and the second triplicate testing on the 200 whole blood samples stored at +4°C (WB4) and at−20°C (WB20)

From: Stability of unfrozen whole blood DNA for remote genotypic analysis of HIV-1 coreceptor tropism

First singlicate test

Second triplicate test

No. of cases

% of cases

WB4

WB20

WB4

WB20

Positive

Positive

NA

NA

133

66.5

Positive

Negative

NA

Positive

19

9.5

Positive

Negative

NA

Negative

3

1.5

Negative

Positive

Positive

NA

16

8.0

Negative

Positive

Negative

NA

3

1.5

Negative

Negative

Positive

Positive

7

3.5

Negative

Negative

Positive

Negative

3

1.5

Negative

Negative

Negative

Positive

1

0.5

Negative

Negative

Negative

Negative

15

7.5

  1. NA, not applicable (second triplicate test run only on samples which were negative in first singlicate test).