Table 1 Primers used in multi-locus sequence typing of Fusarium isolates collected from patients and the environment
Locus | Gene product (size in bp) | Primer | Sequence 5’-3’† | PCR | Sequencing |
|---|---|---|---|---|---|
EF-1α | Translation elongation factor 1 alpha (716) | EF1 | ATGGGTAAGGARGACAAGAC | x | |
EF2 | GGARGTACCAGTSATCATG | x | |||
EF3 | GTAAGGAGGASAAGACTCACC | x | |||
EF22T | AGGAACCCTTACCGAGCTC | x | |||
rRNA | Nuclear ribosomal rRNA (986) | ITS5 | GGAAGTAAAAGTCGTAACAAGG | x | x |
ITS2 | GCTGCGTTCTTCATCGATGC | x | x | ||
ITS3 | GCATCGATGAAGAACGCAGC | x | x | ||
ITS4 | TCCTCCGCTTATTGATATGC | x | x | ||
NL4 | GGTCCGTGTTTCAAGACGG | x | x | ||
RPB2 | RNA Polymerase beta subunit (1738) | 5f2 | GGGGWGAYCAGAAGAAGGC | x | |
7cr | CCCATRGCTTGYTTRCCCAT | x | |||
7cf | ATGGGYAARCAAGCYATGGG | x | |||
11ar | GCRTGGATCTTRTCRTCSACC | x | |||
F2F | AAAGCTCGCCAAGCCCCGTC | x | |||
F1R | GTCCTCTGGTGGCTCCGCCT | x | |||
40-2f | CAAAAACCTCTGGCGACAAC | x | |||
40-r | AGCTTGCGTCCAGTATGACC | x |