Vaginal microbiome in women from Greenland assessed by microscopy and quantitative PCR

BMC Infectious Diseases

Table 2 Sensitivity and specificity before (qualitative detection ¹ ) and after (quantitative detection ² ) applying ROC curve analysis

Species	Qualitative detection¹				Quantitative detection²					AUC
	Se (%)	95% CI	Sp (%)	95% CI	Se (%)	95% CI	Sp (%)	95% CI	Cut-off/ml	Size (%)	95% CI
Atopobium vaginae *	100	95-100	25	15-36	95	88-99	92	83-97	≥55556	97	95-99
Sneathia sanguinegens	75	64-84	86	76- 93	67	56-77	97	90-100	≥14127	85	79-90
Leptotrichia amnionii*	86	76-93	59	47- 70	80	69-88	97	90-100	≥1746	89	83-94
BVAB 1	67	56-77	68	57-79	54	43-66	93	85-98	≥9841	75	68-82
BVAB 2*	91	83-96	79	68-88	87	78-94	97	90-100	≥3492	94	90-98
BVAB 3	66	54-76	86	76-93	66	54-76	92	83-97	≥95	80	74-86
BVAB-TM7	34	24-46	100	95-100	34	24-46	100	95-100	≥2698	67	62-72
Megasphaera type 1*	86	76-93	59	47-70	81	71-89	99	93-100	≥41111	88	82-94
Megasphaera type 2	32	22- 43	93	85-98	28	18-39	100	95-100	≥1111	63	58-69
Eggerthella-like bacterium*	95	88-99	36	25-48	78	68-87	96	88-99	≥297937	91	86-96
Mobiluncus curtisii	25	16-36	99	93-100	25	16-36	99	93-100	≥794	62	57-67
Mobiluncus mulieris	20	12-31	93	85- 98	15	8-25	99	93-100	≥166349	57	52-62
Gardnerella vaginalis*	100	95-100	3	0.3-10	87	78-94	92	83-97	≥4803175	95	91-98
Mycoplasma hominis	63	52-74	85	75-92	58	47-69	96	88-99	≥3968	78	71-84
Ureaplasma parvum	51	39-62	49	37- 61	28	18-39	75	64-85	≥31746	50	42-59
Ureaplasma urealyticum	18	10-28	90	81-96	14	7-24	95	87-98	≥22381	54	49-59
Finegoldia magna	63	52-74	59	47-70	63	52-74	59	47-70	≥159	61	52-69
Prevotella spp.*	97	91-100	4	0.8-12	95	88-99	96	88-99	≥888889	96	92-100

¹Presence versus absence as determined by qPCR.
²Presence (≥ cut-off/ml) versus absence (< cut-off/ml) with cut-off determined by ROC curve analysis.
A total of 79 participants with Nugent III and 73 with Nugent I were used for the evaluation. Intermediate flora not included. L. iners is excluded from the table because it acts as a negative predictor for BV.
*Bacterial species with area under the ROC curve (AUC) > 85%.

ISSN: 1471-2334