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Table 3 Biofilm formation of serial isolates recovered from patients with clinical relapse involving clones identical to the initial isolate

From: Biofilm formation by clinical isolates and the implications in chronic infections

Patient Bacterium Isolate Source Days after initial isolate PFTa Biofilm formerb
1 A. baumannii 1 Deep tissue 0 1 Yes
   2 Deep tissue 3 1 Yes
   3 Bone 8 1 Yes
   4 Deep tissue 68 1 Yes
   5 Deep tissue 70 1 Yes
   6 Deep tissue 127 1 Yes
2 P. aeruginosa 1 Blood 0 1 Yes
   2 Blood 1 1 Yes
   3 Blood 17 1 Yes
   4 Deep tissue 21 1 Yes
   5 Pleural fluid 25 1 No
   6 Deep tissue 29 1 Yes
   7 Blood 36 1 Yes
   8 Deep tissue 42 1 Yes
   9 Respiratory tract 43 1 Yes
   10 Blood 46 1 No
   11 Respiratory tract 49 1 Yes
   12 Blood 51 1 Yes
3 P. aeruginosa 1 Groin swab 0 A Yes
   2 Superficial tissue 332 B No
   3 Superficial tissue 332 B Yes
   4 Deep tissue 522 A Yes
   5 Deep tissue 522 A Yes
4 P. aeruginosa 1 Deep tissue 0 19 Yes
   2 Deep tissue 158 19 Yes
5 MRSA c 1 Deep abscess 0 USA300 Yes
   2 Deep abscess 0 USA300 Yes
   3 Superficial abscess 16 USA300 Yes
   4 Nares swab 105 USA300 Yes
   5 Nares swab 161 USA300 Yes
   6 Nares swab 202 USA300 Yes
   7 Superficial fluid 224 USA300 Yes
   8 Superficial tissue 247 USA300 Yes
   9 Deep tissue 248 USA300 Yes
  1. a Pulsed-field type (PFT).
  2. b Strains with Crystal Violet absorbance greater than or equal to that of Staphylococcus epidermidis ATCC 12228 (positive control) were considered to be positive for biofilm formation.
  3. c Methicillin-resistant S. aureus.