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Table 4 Median Ct values and Ct value differences by real-time PCR

From: Comparison of rectal swabs and faeces for real-time PCR detection of enteric agents in Rwandan children with gastroenteritis

 

Median Ct valuea

dCtb, mean (SE)

P valuec

 

Rectal swab

Faeces

  

ETEC-eltB

32.0/37.5

28.5/37.4

2.28 (0.48)

<0.0001

ETEC-estA

27.7/36.2

25.8/40.1

2.99 (0.48)

<0.0001

E. coli bfpA

27.6/34.2

26.9/37.5

2.10 (0.49)

<0.0001

E. coli eae

34.8/38.1

33.5/38.5

0.85 (0.47)

0.0743

Shigella ipaH

29.4/37.8

28.1/36.7

0.91 (0.58)

0.1245

Campylobacter

32.6/35.8

33.2/37.4

-0.002 (0.59)

1.00

Cryptosporidium

36.2/39.9

34.1/40.9

2.23 (0.76)

0.008

Rotavirus

22.5/41.2

20.9/36.2

2.25 (0.61)

0.0008

Norovirus GG2

30.3/38.8

29.3/33.8

1.45 (0.78)

0.08

Adenovirus

34.0/39.7

35.9/39.9

-0.48 (0.39)

0.21

  1. aConcordant/discordant, i.e. reactive in both rectal swab and faeces/reactive in either rectal swab or faeces. Negative results excluded.
  2. bdCt = Ct in rectal swab–Ct in faeces. For samples reactive in both rectal swab and faeces.
  3. cBy paired t test.
  4. ETEC, enterotoxinogenic E. coli. GG2, genogroup 2.