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Table 2 DLRPA for the detection of M. tuberculosis from sputum samples

From: Phage lysin to control the overgrowth of normal flora in processed sputum samples for the rapid and sensitive detection of Mycobacterium tuberculosis by luciferase reporter phage assay

Culture grade on LJ

No. of samples

LRP

Early time of detection (days)@

Average TTD (in days)

Pos (%)

Neg

Cont

7

9

14

21

LRP

LJ

Negatives

48

8 (16.6)

34

6

0

1

1

6

19

NA

Colonies

9

6 (66.6)

3

0

1

0

0

5

19

38.5

1+

13

11 (84.6)

2

0

3

1

1

6

15.45

29.27

2+

26

24 (92.3)

2

0

8

8

5

3

10.88

20.41

3+

22

22 (100)

0

0

17

2

3

0

8.14

14

NTM

10

0

5

5

0

0

0

0

NA

23.1

Cont

1

0

0

1

0

0

0

0

NA

NA

  1. Pos: Positives; Neg: Negatives; Cont: Contaminated; NTM: Non-tuberculous mycobacteria; TTD: Time-to-detection; NA: Not applicable; LJ: Petroff’s culture; Colonies: 1–19 colonies on LJ; 1+: 20–100 colonies; 2+: >100 colonies; 3+: confluent growth of M. tuberculosis.
  2. @ Number of samples detected at early time (i.e. between 7 to 21 days) by D.