Molecular characterization of T cell receptor beta variable in the peripheral blood T cell repertoire in subjects with active tuberculosis or latent tuberculosis infection

Table 5 Representative amino acid sequences of monoclonal TCRBV families within PBMCs and CD4 ⁺, CD8 ⁺ T cell populations of LTBI subjects ^a

Subjects^b	Vbeta		CDR3	BJ		Ratio^c
J24 (CD4+)	BV24	AAMYLCATS	SDRVSPL	HFGNGTRLTVTED	1.6	22/25
J23	BV20	SGFYLCAWS	VQVGWGETQ	YFGPGTRLLVLED	2.5	12/21
J24 (CD4+)	BV20	SGFYLCAWS	TGTGHSPL	HFGNGTRLTVTED	1.6	21/25
J28 (CD8+)	BV20	SGFYLCAWS	TGTGHSPL	HFGNGTRLTVTED	1.6	20/24
J38	BV20	SGFYLCAWS	TGTGHSPL	HFGNGTRLTVTED	1.6	22/25
J32 (CD4+)	BV14	SLYFCASS	PGTTQETQ	YFGSGTRLLVLED	2.5	18/23
J25	BV14	SLYFCASS	PGTTQETQ	YFGPGTRLTVLED	2.5	15/23
J28 (CD8+)	BV14	SLYFCASS	RHTGGTEA	FFGQGTRLTVVED	1.1	19/30
J29	BV13.2	TSVYFCASS	YDENEQ	FFGPGTRLTVLED	2.1	16/22
J31 (CD4+)	BV13.2	TSVYFCASK	TKPVGEQ	FFGPGTRLTVLED	2.1	15/23
J42	BV13.1	TSVYFCASS	YSAGGPNEQ	FFGPGTRLTVLED	2.1	18/25
J34 (CD4+)	BV12	TSVYFCAIR	KQGDSYEQ	YFGPGTRLTVTED	2.7	17/23
J35	BV12	TSVYFCASR	VRLITNYGY	TFGSGTRLTVVED	1.2	24/24
J39 (CD4+)	BV12	TSVYFCATA	GGAPGQPQ	HFGDGTRLSILED	1.5	16/24
J23	BJ5.1	SALYLCASS	LDYSGNTI	YFGEGSWLTVVED	1.3	22/27
J25 (CD8+)	BJ5.1	SALYLCASS	LDYSGNTI	YFGEGSWLTVVED	1.3	22/27
J30	BJ5.1	SALYLCASS	LDYSGNTI	YFGEGSWLTVVED	1.3	22/25
J24	BV3	SMYLCASG	PNYYEQ	YFGPGTRLTVTED	2.7	23/25

^aWhen the GMSP profile of a TCRBV family displayed a single peak, the corresponding PCR products re-amplified, and the CDR3 were sequenced after cloning.
^bThe T cell subsets (CD4⁺ or CD8⁺) for analysis are indicated in brackets.
^cThe amino acid sequences of the max rate of TCRBV families are shown.

ISSN: 1471-2334