SDS-PAGE and Western blot analysis of total extract of
A. Twenty micrograms of total extract of M. leprae were separated by 10-20% gradient SDS-PAGE and the gel was stained with silver nitrate (lane 2). Molecular weight marker is shown in the lane 1. B. The proteins were transferred to PVDF membrane and blots were probed with 1 μg/mL of MB patients’ total IgG (lane 1), 60 ng/mL of MB patients’ antigen-specific IgG (lane 2) and 1 μg/mL of PB patient’ total IgG (lane 3). Detection of the reaction was performed with anti-human IgG (Fc-specific)-peroxidase antibody (1:30000) and chemiluminescence.