Figure 2

KIR3DS1 and KIR3DL1 mRNA levels of different groups. Shown are KIR transcript levels reflecting the relative expression of KIR3DS1 and KIR3DL1 mRNA normalized to GAPDH mRNA in total RNA preparations. Reported values are ratios of KIR and GAPDH concentrations. The lines denoted the medians. (A) KIR3DS1 mRNA levels of the “high CD4⁺ T cell count” group experienced a significant increase compared to KIR3DS1 mRNA levels in the “low CD4⁺ T cell count” group (P = 0.01). (B) KIR3DS1 mRNA levels of the “high viral load” group experienced a significant decrease compared to those in the “low viral load” group (P = 0.006). (C) Levels of KIR3DS1 mRNA in TPs were significantly lower than in LTNPs (P = 0.003). (D) KIR3DL1 mRNA levels in the “high CD4⁺ T cell count” group experienced a significant decrease compared to those in the “low CD4⁺ T cell count” group (P = 0.041). (E) There was no statistical difference between levels of KIR3DL1 mRNA in the “high viral load” group and the “low viral load” group (P = 0.067). (F) KIR3DL1 mRNA levels of TPs were significantly higher than those of LTNPs (P = 0.023). (G) The electrophoretogram was shown to demonstrate the RT-PCR specificity. The PCR reaction only generates a single band. Lane 1, 2 and 3 were for KIR3DL1, and lane 4, 5 and 6 were for KIR3DS1 using different primer concentration.