Detection of dengue viruses using reverse transcription-loop-mediated isothermal amplification

BMC Infectious Diseases

Table 1 RT-LAMP primers used for the rapid detection of DENV

Primer^a,b	Sequence (5′ → 3′)
F3/134	CAAACCGTGCTGCCTGT
F3/2	TGAGTAAACTATGCAGCCTGT
B3/123	ACCTGTTGATTCAACAGCACC
B3/4	ACCTGTTGGATCAACAACACC
FIP/123	AGGGGTCTCCTCTAACCRCTAGTCTTTCAAACCRTGGAAGCTGTACGC
FIP/4	AGGGGTCTCCTCTAACCRCTAGTCTTTTTTGCCACGGAAGCTGTACGC
BIP/123	ACAGCATATTGACGCTGGGARAGACGTTCTGTGCCTGGAATGATGCTG
BIP/4	ACAGCATATTGACGCTGGGARAGACGCTCTGTGCCTGGATTGATGTTG
BLP/1234	CAGAGATCCTGCTGTCTC

^a F3 forward outer primer, B3 backward outer primer, FIP forward inner primer, BIP backward inner primer, BLP backward loop primer.
^bNumber (1–4) after the slash (/) represents DENV serotype (e.g. F3/134, forward outer primer for DENV-1, -3 and −4).

ISSN: 1471-2334