Table 1 List of primers used to amplify streptococcal superantigen genes, 16S ribosomal RNA (rRNA), and emm genes
Name | Sequence 5′-3′ | Source |
|---|---|---|
SpeA forward | AAAGTTGCCATCTCTTGGTTC | Sigma genosys |
SpeA reverse | CAAGAGGTATTTGCTCAACAAGAC | Sigma genosys |
SpeC forward | TTTGAGCAGGCGTAATTCCT | Sigma genosys |
SpeC reverse | TTCAACGACACACACATTAAACA | Sigma genosys |
SpeG forward | ACCCCATGCGATTATGAAAA | Sigma genosys |
SpeG reverse | GGGAGACCAAAAACATCGAC | Sigma genosys |
SpeH forward | ATTCCAATGTTGTTCAAGCAAA | Sigma genosys |
SpeH reverse | TGAGCGGTTACTTTCGGTTT | Sigma genosys |
SpeI forward | TCCGCCATTTTCAGGTAGTT | Sigma genosys |
SpeI reverse | TTTCCTTCCTCAAAGCCAGA | Sigma genosys |
SpeJ forward | GCTCTCGACCTCAGAATCAA | Sigma genosys |
SpeJ reverse | CTTTCATGGGTACGGAAGTG | Sigma genosys |
SpeK forward | CAAACAAGGAACGCAATTGAT | Sigma genosys |
SpeK reverse | GTGTCTAATGCCACCGTCT | Sigma genosys |
SpeL forward | ATAAGTCAGCACCTTCCTCTTTC | Sigma genosys |
SpeL reverse | AAATCTCCCGTTACCTTCCA | Sigma genosys |
SpeM forward | AACTTCTTCTTCCTTAAAGCGTCT | Sigma genosys |
SpeM reverse | TGCTGTGTTGGTTAATAGCGA | Sigma genosys |
SmeZ forward | TTTCTCGTCCTGTGATTGGA | Sigma genosys |
SmeZ reverse | AATGGGACGGAGAACATAGC | Sigma genosys |
SSA forward | ACAGGTCAGCTTTTACAGCA | Sigma genosys |
SSA reverse | GGGCATCATATCGTACCAAA | Sigma genosys |
16S rRNA forward | AGAGTTTGATCCTGGCTCAG | Invitrogen life technologies |
16S rRNA reverse | AAGGAGGTGATCCAGCCGCA | Invitrogen life technologies |
emm genotyping primer forward | TATTCGCTTAGAAAATTAA | Invitrogen life technologies |
emm genotyping primer reverse | GCAAGTTCTTCAGCTTGTTT | Invitrogen life technologies |