Analysis of mutations in the E6 oncogene of human papillomavirus 16 in cervical cancer isolates from Moroccan women

Table 2 Characterization of HPV16 E6 variants and their lineage classification

	HPV 16 variants		N	T109C	A131G	G132T	C143G	G145T	C285G	T286A	A289G	T295G	T325C	C335T	T350G	A403G	A442C	Predicted substitution
	HPV16 Reference			T	A	G	C	G	C	T	A	T	T	C	T	A	A
Lineage	Sub-lineage	Class/sub-class
EUR	Ep	E-T350	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-
	E-350G	E-G350	16	-	-	-	-	-	-	-	-	-	-	-	G	-	-	L83V
		E-C442/G350	40	-	-	-	-	-	-	-	-	-	-	-	G	-	C	L83V/E113D
		E-G350/T145	1	-	-	-	-	T	-	-	-	-	-	-	G	-	-	Q14H/L83V
AFR	Af1	Af1-d/G295	5	-	G	-	G	T	-	a	g	G	-	T	G		-	R10G/Q14D/D64E/H78Y/L83V
	Af1	Af1-d/G295/C325	2	-	G	-	G	T	-	a	g	G	c	T	G	-	-	R10G/Q14D/D64E/H78Y/L83V
	Af2	Af2-a/C109/G403	13	c	-	T	G	T	-	a	g	-	-	T	-	g	-	R10I/Q14D/H78Y
		Af2-a/G285	1	-	-	T	G	T	G	a	g	-	-	T	-	g	-	R10I/Q14D/A61G/H78Y
		Af2a/r	10	-	-	T	G	T	-	a	g	-	-	T	-	g	-	R10I/Q14D/H78Y
NA	NA1	NA1	9	-	-	-	-	-	-	a	g	-	-	T	-	g	-	H78Y
		NA1	2	-	-	-	-	-	-	a	g	-	-	T	G	-	-	H78Y/L83V
		NA1-b/r	1	-	-	-	-	T	-	a	g	-	-	T	G	-	-	Q14D/H78Y/L83V
N*				13	7	24	31	33	1	43	43	7	2	43	67	33	40

Sequence alterations relative to the E6 open reading frame (ORF) of the reference HPV16 sequence [35]. For subclasses, the number and the letter represent the nucleotide change at that position. Lower case represents a nucleotide change at that position without an amino acid change. Capital letters indicate variants with an amino acid change. In the ‘Predicted substitution’ column, the letter preceding the amino acid position refers to the reference HPV16 sequence and the letter after it refer to the substitution. Dashes indicate no variation. * Total cases harboring the point mutation. EUR: European lineage, AFR: African lineage, NA: Nord American lineage.

ISSN: 1471-2334