Figure 2

Lung cytokine levels in mice infected with aerosolized L. pneumophila . Cytokine levels in lung homogenates of mice infected with aerosol infection by Lp Phil-1. IL-1β (A), CXCL1 (B), CXCL2 (C), CCL2 (D), TNF (E), IL-6 (F), GM-CSF (G), and IFN-γ (H) were measured by fluorescent bead immunoassay at 4 hours and 24 hours in untreated controls (diagonal hatched bars), WT (C57/Bl6) (black bars), Nlrc4−/− (white bars), and Nlrc4−/−/Tlr5−/− (horizontal hatched bars) mice. Cytokine levels from uninfected controls are shown in (Con) lane at 4 h. Data is from n = 4-8 mice except uninfected control (n = 1). * = p < 0.05, † = p < 0.01 as determined by one-way ANOVA test.