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Table 1 Detection of recombinant SsTPx in infected S. scabiei serum, negative serum and controls by dot-ELISA

From: Characterisation and analysis of thioredoxin peroxidase as a potential antigen for the serodiagnosis of sarcoptic mange in rabbits by dot-ELISA

Groups (n = 203)

Observer 1 number

Sensitivity or specificity

Observer 2 number

Sensitivity or specificity

Kappa index*

Proportion agreement(PA)

+

–

+

–

S. scabiei infected-(n=85)

81

4

95.3%

80

5

94.1%

0.882

98%

Total uninfected (n=118)

7

111

94.1%

8

110

93.2%

0.928

99%

Negative (n=94)

0

94

100%

0

94

100%

1.0

100%

E.stiedae (n=6)

0

6

100%

0

6

100%

1.0

100%

T.pisiformis (n=6)

0

6

100%

0

6

100%

1.0

100%

P. cuniculi (n=12)

7

5

68.3%

8

4

66.7%

0.823

91%

  1. *Strength of agreement: K index <0, poor; 0–0.20, slight; 0.21–0.4, fair; 0.41–0.6, moderate; 0.61–0.80, substantial; 0.81–1.0; almost perfect.