A rooted phylogenetic tree of 53 complete S sequences of HBV obtained from Sudanese liver disease patients (shaded in box) with 104 reference HBV sequences, using neighbour-joining. Bootstrap statistical analysis was performed using 1000 datasets, indicated as percentages on the nodes. The sequences are labeled by their accession numbers and country. [AUS, Australia; CMR, Cameron; CAN, Canada; CAR, Central African Republic; CHN, China; COV, Côte d’Ivoire; DRC, Democratic Republic of Congo; EGP, Egypt; EST, Estonia; FR, France; GHN, Ghana; GER, Germany; HAI, Haiti; IND, India; IRN, Iran; ITA, Italia; JAP, Japan; KAZ, Kazakhstan; LEB, Lebanon; MAD, Madagascar; MLY, Malaysia; MLW, Malawi; NAM, Namibia; NIG, Niger; PAK, Pakistan; PAP, Papua New Guinea/Indonesia; POL, Poland; RUS, Russia; RWD, Rwanda; SRB, Serbia; SOM, Somalia; SA, South Africa; SPN, Spain; SDW, Sweden; TNZ, Tanzania; TAJ, Tajikistan; TUN, Tunisia; TUR, Turkey; UGN, Uganda; UK, United Kingdom; USA, United States; UZK, Uzbekistan ]. The letters, A, D and E, represent the genotypes and the numbers the subgenotypes. A rooted phylogenetic tree of the complete genome of SDAC 031 (shaded) relative to 17 reference HBV sequences, using neighbour-joining is shown in the left hand box. When the complete genome was analyzed SDAC031 clustered with D6 whereas when the S region alone was analyzed it clustered with D4.