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Table 5 Capacity of the (H6-ED3-PhoA) 2 reagents to discriminate between serums infected by different DENV serotypes in a MAC-ELISA

From: Cross-reactivities between human IgMs and the four serotypes of dengue virus as probed with artificial homodimers of domain-III from the envelope proteins

(−) Serums R1 R2 R3 R4
DENV1 na 0.52 ± 0.09 0.55 ± 0.10 0.57 ± 0.08
DENV2 0.78 ± 0.08 na 0.68 ± 0.08 0.70 ± 0.07
DENV3 0.84 ± 0.07 0.72 ± 0.08 na 0.80 ± 0.07
DENV4 0.87 ± 0.06 0.85 ± 0.05 0.81 ± 0.07 na
Other DENV 0.84 ± 0.06 0.72 ± 0.06 0.70 ± 0.06 0.69 ± 0.06
All others 0.90 ± 0.05 0.81 ± 0.04 0.73 ± 0.06 0.66 ± 0.06
  1. R1 to R4, (H6-ED3-PhoA)2 dimers of serotypes DENV1 to DENV4, respectively; na, non applicable. The serotype of the infecting DENV for the (+) serums was always identical with the serotype of the reagent. The first column gives the serotype of the infecting DENV for the (−) serums. Other DENV, all DENV serotypes except the serotype of the reagent. All others, all serums (infected and uninfected) except those infected by the same DENV serotype as the reagent. Each entry gives the accuracy and associated standard error of a MAC-ELISA that used the Ri reagent (i = 1, …, 4) as an antigen and was evaluated on the (+) and (−) serums. Note that the expressions (+) serums and (−) serums refer to the discrimination under statistical analysis and not to the infected or uninfected character of the serums.