Table 2 Primers used in real-time PCR analysis of T.gondii IVIAT identified genes by IgG
GenBank ID/accession no. | Primer | |
|---|---|---|
| Â | Orientation sequence | |
** β -Actin | *F | 5′-TCACACTGTGCCCATCTACGA-3′ |
*R | 5′-GTGGTGAAGCCGTATCCTCTCT-3′ | |
7901108 | F | 5′-AAAGATGCTCCACGCCAACT-3′ |
R | 5′-GTTTGTCCACGTTGCTTCCA-3′ | |
7901478 | F | 5′-TGAGCCAGAAACCTGACAAACA-3′ |
R | 5′-ATCGCCTTTTCCGCTGATTT-3′ | |
7894322 | F | 5′-TCCTAAAGCTCGGCGGAATT-3′ |
R | 5′-CGGTTTGGGCTTTCCAGTTT-3′ | |
7894761 | F | 5′-AGGCGTTTCAACCGACTCTGT-3′ |
R | 5′-ACCTGTGGCACAACAAGACATC-3′ | |
7897485 | F | 5′-CAAGCGTGAGTTCGAGCAATAC-3′ |
R | 5′-GGCTTCCCATTTCGGTCATA-3′ | |
7899395 | F | 5′-TCGTGGTGGACAAGGTATAGGAT-3′ |
R | 5′- CCATAGTTGTCGGAAGAGTCGTT-3′ | |
7895435 | F | 5′- GGGAAAGCATCTGGCTTCAA-3′ |
R | 5′- GAGGAACGGAAAGTAGCGGAAT-3′ | |
7895096 | F | 5′-CTCGAAACGATCGCTGCTTT-3′ |
R | 5′-ACTCCCGCAGAGTTCAAGACTT-3′ | |
7895077 | F | 5′-CCTTCCACAAGTTGGCACAGA-3′ |
R | 5′-CAGTAGCTCCTCAAGCAGTGACA-3′ | |
7897605 | F | 5′-GATGATCGAAGCTCGCAAGTG-3′ |
R | 5′-TAGACAAGGTGCCGCATCAAC-3′ | |
7899217 | F | 5′-GTTCCTCGAGCTTTCCAACCT-3′ |
R | 5′-AGAGCTGCATCTGGAGCAGAA-3′ | |
AM055942.3 | F | 5′-GAGGCACCGCTTCTTCTATACTG-3′ |
R | 5′-TGGGATCGTGTAAGCCATAGC-3′ | |
7894058 | F | 5′-TTTCCCTGCGGAGCAGTCT-3′ |
R | 5′-CGCCTGCAACCTGAGATGA-3′ | |
7893896 | F | 5′-GCGAAAGGTCTGGAACATTTG-3′ |
R | 5′-GGCAGGTAGGAAAGGAAATCGT-3′ | |
7899266 | F | 5′-GGCACTTGTCAATGCAGAACA-3′ |
R | 5′-CTAATTGACCGGTGCACGAAA-3′ | |
7893521 | F | 5′-GGAGGTCATCCTGTGGACTCA-3′ |
R | 5′-GGCTCACGACTTTCACCTTCA-3′ | |
7893531 | F | 5′-CGATACATGCGGCAGAGTCA-3′ |
R | 5′-TGCTGGCTCGTCACATTCAG-3′ | |
7896164 | F | 5′-ATCTCGGGAGTCTTCAGCAGACT-3′ |
R | 5′-GACGGCTGTTTCTCGACTTTTC-3′ | |
7896097 | F | 5′-TGTTGCTACCATGGGCCATT-3′ |
R | 5′-TGTATCTGTGCCGGTCTTCGT-3′ | |
7901507 | F | 5′-CGTATTCAAGCGCTGTTCGA -3′ |
R | 5′-GAGACGATTGGCAGCGAATT -3′ | |
7894761 | F | 5′-AACCGACTCTGTCGCATCTGT-3′ |
R | 5′-CAAACCTGTGGCACAACAAGA-3′ | |
7900407 | F | 5′-GCCTTCCTCGTAACCCAACTT-3′ |
R | 5′-AAGCTTGCCAGAACGACGAA-3′ | |
7897848 | F | 5′-CTGATTCGGGCACTGATTGA-3′ |
R | 5′-GACGCGAAAAAGAGGGCATA-3′ | |
7900113 | F | 5′-CGAGTTCTTTCGCGCTTTCT-3′ |
R | 5′-ATGGTTGGCAGACGGTCAAG-3′ | |
7899268 | F | 5′-TCAGCTCGGAAGCACAGAATC-3′ |
R | 5′-ACGTGTCAGGCGGTGAATACT-3′ | |
7893794 | F | 5′-TCCCGAAGGGAAAAAGTAGTCATA-3′ |
R | 5′-TTTGTCTTGGACTTCCGTATACCA-3′ | |
7893464 | F | 5′-CGAACCCTGCCAATTCTCAT-3′ |
R | 5′-TCCATCCCCTCAGCAAAGTT-3′ | |
7899477 | F | 5′-CTGTGTAAGGCGAATTTCTTTCG-3′ |
R | 5′-AGAAGAGAATCTGGCACGATGTG-3′ | |
AY424892.1 | F | 5′-CAGGAAGCCATGCACACTGT-3′ |
|  | R | 5′-GGCGACTCGATCCATTCCTA-3′ |