Compartmentalized, functional role of angiogenin during spotted fever group rickettsia-induced endothelial barrier dysfunction: evidence of possible mediation by host tRNA-derived small noncoding RNAs

Table 2 The most abundantly cloned tRF-5s.

	Sequence	Parental	Relative cloning frequency (‰)
			Mock	Ricketsia infection
			Mock	day 1	day 3
tRF5-ValGTG	GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT*	tRNA-Val GTG, tRNA Val GTY	3.984	6.494	12.950
tRF5-GlyGCC	GCATTGGTGGTTCAGTGGTAGAATTCTCGC*	tRNA-Gly-GYY, tRNA-Gly-GGG	4.767	5.734	7.638
tRF5-GlyGCC (A to C)	GCCTTGGTGGTTCAGTGGTAGAATTCTCGC	tRNA-Gly-GYY, tRNA-Gly-GGG	2.977	3.954	4.791
tRF5-GluCTC	TCCCTGGTGGTCTAGTGGTTAGGATTCGGC	tRNA-Glu-GAG	0.700	0.501	1.108
tRF5-LysCTT	TCCCTGGTGGTCTAGTGGTTAGGATTCGGC	tRNA-Lys-AAG	0.429	0.577	1.144

For individual tRF-5 sequences, their relative cloning frequencies were calculated as described in the Figure 6 legend. Based on the value (expressed in ‰ [1 per 1,000]), the most abundantly cloned tRF-5s were selected and tabulated. Among the five tRF-5s, two (tRF5-ValGTG and tRF5-GlyGCC: highlighted by asterisks in their sequences) were chosen for further study. “tRF5-GlyGCC (A to C)” has been assigned as a tRF-5, because we failed to find its identical sequence in any database and it mapped to tRF5-GlyGCC perfectly, except that the third nucleotide was C while the correct one in the mouse genome database is A.

ISSN: 1471-2334