Figure 4

conorii infection-initiated cytoplasmic translocation of exogenous rANG and enhanced para-endothelial hyperpermeability at 72 hrs p.i., this effect can be attenuated by co-administration of neomycin with rANG. Endothelial cells were seeded on type I rat-tail collagen-coated polycarbonate transwell filters and infected with R. conorii at an MOI of 10 in triplicate, or mock infected. After 24 and 72 hr, HUVECs were exposed to ANG or co-administration of neomycin with ANG for two hrs. FITC-dextran was added to the upper chamber medium, and the presence of FITC dextran in the lower chamber was quantified after 1 hr. The results are expressed as the fold-increase in monolayer permeability over basal permeability levels (* P< 0.05). Experiments were performed in replicates of four.